摘要
目的探究2011~2020年大连地区无偿献血人群的HIV-1基因型分布和变化趋势,为临床用血安全、艾滋病防治提供基础数据。方法提取大连市2011~2020年确认HIV感染的无偿献血者标本HIV RNA,并对其pol片段进行扩增、测序,分析标本HIV-1基因型和系统进化关系。结果共完成174份HIV感染标本的基因型分析。大连无偿献血人群HIV-1的流行基因型包括CRF01_AE(69.5%)、CRF07_BC(17.2%)、B(5.2%)、CRF02_AG(2.9%)、C(1.1%)、CRF55_01B(1.1%)、CRF08_BC(0.6%)、CRF59_01B(0.6%)、CRF79_0107(0.6%),以及2例未确定基因型(1.1%)。各基因型分布与全国数据有显著区别;在此10年间,CRF01_AE占比整体呈现波动下降趋势,而CRF07_BC占比明显上升。CRF02_AG感染人群与本地主要基因型相比,女性感染者更多(占比分别为40.0%、2.4%),年龄偏大(中位数分别为35岁、26岁),并且教育水平更低(主要为初中及以下学历)。96.7%的本地CRF01_AE型标本,汇聚于东北地区MSM人群流行的传播簇。结论大连地区无偿献血人群HIV-1型有其独特的分子流行特征,CRF01_AE和CRF07_BC此消彼长的趋势滞后于全国总体数据。对重点人群的征询服务和血液安全宣传、教育亟待加强。
Objective To investigate HIV-1 subtype distribution and trend among Dalian blood donors between 2011 and 2020,in order to provide information to improve blood safety and HIV prevention.Methods HIV RNA was purified from plasma samples of Dalian blood donors with confirmed HIV infection,which were collected between 2011 and 2020.The HIV pol gene was amplified and sequenced.HIV-1 subtypes were determined by phylogenetic analysis.Results HIV RNA was successfully genotyped in 174 samples from HIV-infected donors.The main subtypes among Dalian blood donors were CRF01_AE(69.5%),CRF07_BC(17.2%),B(5.2%),CRF02_AG(2.9%),C(1.1%),CRF55_01B(1.1%),CRF08_BC(0.6%),CRF59_01B(0.6%)and CRF79_0107(0.6%).There were still 2 cases(1.1%)unclassified.Significant difference was observed when comparing with the published national data.The prevalence of CRF01_AE strains decreased over the years,while CRF07_BC increased significantly.CRF02_AG carriers differed from donors infected with other HIV sub-types by being mostly females(40.0%vs.2.4%),aged(median:35y vs.26y)and lower educational background(junior school degree or below).And 96.7%of local CRF01_AE cases were related to HIV strains,which were reported to circulate in Northeast China and in the MSM population.Conclusion HIV-1 among Dalian blood donors had unique molecular epidemiology and the trends of 07_BC increasing and 01_AE decreasing lagged behind the overall national data.Donor education on blood safety and consultation services to high risk group before donation still need improvement.
作者
郭笑寒
刘笑春
王颖颖
臧亮
Daniel Candotti
邓雪莲
GUO Xiaohan;LIU Xiaochun;WANG Yingying;ZANG Liang;Daniel Candotti;DENG Xuelian(Dalian Blood Center,Dalian 116001,China;Department of Virology,Henri Mondor Hospital,AP-HP,University of Paris-Est,INSERM U955,IMRB,Créteil,France)
出处
《中国输血杂志》
CAS
2022年第9期954-958,共5页
Chinese Journal of Blood Transfusion
基金
大连市医学科学研究计划项目(2011038)。
