摘要
目的了解岳阳地区无偿献血者丙型肝炎病毒(HCV)感染情况,降低HCV经血液传播风险。方法采用酶联免疫吸附试验(ELISA)和核酸检测技术(NAT),检测无偿献血者血液标本抗-HCV或HCV。结果岳阳地区2020年57015例无偿献血者标本ELISA检测出抗-HCV阳性53例,NAT检测出HCV感染“窗口期”1例。不同血型、性别、年龄无偿献血者抗-HCV阳性率比较,差异均无统计学意义(P>0.05);不同职业无偿献血者抗-HCV阳性率比较,差异有统计学意义(P<0.05)。53例抗-HCV阳性标本经NAT检测,检出HCV阳性14例,阴性39例。对14例HCV阳性标本进行基因分型,1b型6例,2a型1例,3a型1例,6a型6例。结论岳阳地区不同职业无偿献血人群HCV感染情况有差异,HCV型别呈多样性。
Objective To investigate the infection of hepatitis C virus(HCV)in Yueyang among unpaid blood donors and reduce the risk of HCV transmission through blood.Methods The enzyme-linked immunosorbent assay(ELISA)and nucleic acid testing(NAT)were used to detect the anti-HCV or HCV in blood samples of unpaid blood donors.Results Among 57015 unpaid blood donors in Yueyang area in 2020,53 cases of anti-HCV were detected positive by ELISA,and one case of HCV infection"window period"was detected by NAT.There was no significant difference in the positive rate of the anti-HCV among unpaid blood donors of different blood types,gender and age(P>0.05),but there was significant difference among the unpaid blood donors with different occupations(P<0.05).Among the 53 anti-HCV positive samples,14 were positive and 39 were negative by NAT.Genotyping of the 14 HCV-positive samples was performed,including 1b(six cases),2a(one case),3a(one case)and 6a(six cases).Conclusion There are differences in the HCV infection in the unpaid blood donors with different occupations in Yueyang,and the HCV types are diverse.
作者
刘锋
方奎明
黄志翔
吴晓晋
祝青
刘娟
LIU Feng;FANG Kuiming;HUANG Zhixiang;WU Xiaojin;ZHU Qing;LIU Juan(Department of Laboratory Medicine,Yueyang Central Blood Station,Yueyang,Hunan 414000,China)
出处
《现代医药卫生》
2021年第22期3816-3818,共3页
Journal of Modern Medicine & Health
基金
湖南省岳阳市科技局基础研究计划项目(岳市科发〔2020〕21)。
关键词
无偿献血者
丙型肝炎病毒
基因分型
核酸检测
湖南
Unpaid blood donors
Hepatitis C virus
Genotyping
Nucleic acid testing
Hunan
