摘要
为了解银川市接受抗病毒治疗的HIV-1感染者基因亚型和耐药突变特征。收集2020年银川市接受抗病毒治疗的HIV-1感染者血样,其中病毒载量大于400拷贝/mL的样本进行基因型耐药检测,通过巢式聚合酶链反应(PCR)扩增HIV-1 pol基因区片段并测序,对所得序列进行基因分型和耐药突变分析。收集642例血浆样本,50例病毒载量大于400拷贝/mL,基因型耐药检测后获得29例pol区基因序列。17例耐药,3例潜在耐药,总体耐药率为2.65%(17/642)。CRF07_BC和CRF01_AE是耐药株的主要基因亚型。NRTIs类耐药突变中,M184V/I(31.03%)突变发生率最高,其次为K65R(17.24%)。3TC、FTC和ABC耐药发生率最高,均为37.93%(11/29)。NNRTIs类耐药突变中,G190A/S/E、V179D/DE/E突变发生率最高,均为24.14%;K103N/KN(17.24%)次之。EFV和NVP耐药发生率最高,均为58.62%(17/29)。2020年银川市新型重组亚型增加,首次检出CRF79_0107。总体耐药率处于低水平,以NNRTIs耐药为主,需加强耐药监测,严防新型重组毒株的流行。
To investigate the characteristics of HIV-1 genotypes and drug resistance mutations(DRMs)of patients who received antiretroviral therapy(ART)in Yinchuan,China.Plasma samples were collected from HIV/AIDS patients who had received ART in Yinchuan,2020.HIV-1 genotyping drug resistance testing was carried out on patients with viral load>400 copies/mL.The viral HIV-1 pol gene was amplified and sequenced by nested PCR,and sequences were analyzed for HIV-1 subtypes and drug resistance.A total of 642 samples were collected,among which 50 has a viral load>400 copies/mL.Twenty-nine pol gene sequences were acquired,in which 17 cases had drug resistance mutations and 3 cases had potential drug resistance mutations.The drug resistance rate of Yinchuan was 2.65%(17/642).CRF07_BC and CRF01_AE are predominant gene subtypes of resistant strains.Among NRTI DRMs,M184V/I(31.03%)was the most frequent mutation,followed by K65R(17.24%).The drug resistance rates of 3TC,FTC and ABC were highest(37.93%).Among NNRTI DRMs,G190A/S/E and V179D/DE/E were the most frequent mutations,followed by K103N/KN.EFV and NVP showed the highest drug resistance rate of 58.62%(17/29).In 2020,novel HIV-1circulating recombinant forms(CRFs)in Yinchuan were increasing,and CRF79_0107 was identified for the first time.In addition,HIV-1 drug resistance was at a low level,NNRTIs were the main type.It is essential to take steps to strengthen the surveillance of drug resistance and prevent the spread of novel CRFs.
作者
吴玉灵
杨东智
李宗倍
李龙山
裴建新
吴忠兰
WU Yuling;YANG Dongzhi;LI Zongbei;LI Longshan;PEI Jianxin;WU Zhonglan(Ningxia Medical University,Yinchuan 750004,China;Ningxia Center for Disease Control and Prevention,Yinchuan 750000,China;Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control,Yinchuan 750004,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2022年第5期1160-1165,共6页
Chinese Journal of Virology
基金
宁夏重点研发项目(项目号:2018BEG03065),题目:基于深度测序技术研究宁夏地区HIV-1前病毒DNA和血浆RNA耐药现况及准种分子进化情况
2017年宁夏青年拔尖人才项目(项目号:宁人社函[2017]787号)
2020年宁夏回国人员创新团队项目(项目号:宁人社函[2021]5号),题目:核酸定量检测在优化艾滋病一站式早期诊断中的应用。
