摘要
目的:提高虎奶菇菌丝体抗菌肽得率和抑菌率。方法:将抗菌肽的得率和对金黄色葡萄球菌及大肠杆菌的抑菌率作为评价指标,在蛋白酶筛选的基础上,优化虎奶菇菌丝体抗菌肽的提取工艺,并测定其抗菌肽的最低抑菌浓度。结果:碱性蛋白酶酶解制备虎奶菇菌丝体抗菌肽的最佳工艺条件为酶解温度39.5℃、酶解时间3.2 h、pH值11.1,料液比1∶20(g/mL),酶添加量6000 U/g,此条件下虎奶菇菌丝体抗菌肽对金黄色葡萄球菌和大肠杆菌的实际抑菌率分别为74.17%和86.22%,抗菌肽提取率为22.67%。抗菌肽对金黄色葡萄球菌和大肠杆菌的MIC值分别为5.0,2.5 mg/mL。结论:虎奶菇菌丝体抗菌肽具有显著的抑菌活性,尤其是对大肠杆菌的抑菌作用更强。
Objective:To improve the yield and antibacterial rate of antimicrobial peptides from Pleurotus tuber-regium mycelium(PTRM-AMPs).Methods:Taking the yield and the antibacterial rate of PTRM-AMPs against Staphylococcus aureus and Escherichia coli as evaluation indexes,on the basis of protease screening,the extraction process of PTRM-AMPs was optimized,and the minimum inhibitory concentration of PTRM-AMPs was determined.Results:The optimum technological conditions for the preparation of PTRM-AMPs by alkaline protease hydrolysis were as follows:the enzymatic hydrolysis temperature was 39.5℃,the enzymatic hydrolysis time was 3.2 h,the pH value was 11.1,the solid-liquid ratio was 1∶20(g/mL),and the enzyme addition was 6000 U/g.Under these conditions,the actual antibacterial rates of PTRM-AMPs against S.aureus and E.coli were 74.17%and 86.22%respectively,and the yield of PTRM-AMPs was 22.67%.The MIC values of PTRM-AMPs against S.aureus and E.coli were 5.0,2.5 mg/mL respectively.Conclusion:PTRM-AMPs has significant antibacterial activity,especially against E.coli.
作者
张书会
罗璐
孙雪言
马爱民
ZHANG Shu-hui;LUO Lu;SUN Xue-yan;MA Ai-min(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China)
出处
《食品与机械》
北大核心
2022年第8期158-165,共8页
Food and Machinery
基金
国家自然科学基金项目(编号:31772375)。
关键词
虎奶菇菌丝体
抗菌肽
酶解法
抑菌活性
得率
Pleurotus tuber-regium mycelium
antimicrobial peptide
enzymatic hydrolysis
antibacterial activity
yield
