摘要
目的:探究板枣多糖的理化性质、初级结构及抗氧化活性。方法:采用水提醇沉、三氯乙酸脱蛋白、过氧化氢脱色等方法制备板枣多糖,测定其理化指标,利用紫外和红外光谱进行扫描推测可能存在的结构,最后测定其自由基清除能力。结果:板枣多糖中总糖质量分数为52.30%,糖醛酸含量46.22%,酯化度64.70%,是一种酸性果胶多糖;紫外和红外光谱分析表明板枣多糖具有典型的多糖类特征吸收峰,不含蛋白质和核酸,是一种含有α-糖苷键的吡喃糖;板枣多糖主要由半乳糖醛酸、半乳糖、阿拉伯糖、葡萄糖组成。板枣多糖有一定的抗氧化活性,质量浓度为1.0 mg/mL时,其对DPPH自由基、羟自由基和ABTS自由基清除率分别为23.64%,21.01%,13.84%。结论:板枣多糖是以半乳糖醛酸为主的酸性果胶糖,含有α-糖苷键,抗氧化能力较强,可作为潜在的抗氧化剂。
Objective:This study aimed to analyze the physicochemical properties,primary structure and antioxidant activity of polysaccharide from Zizyphus jujuba cv.Banzao.Methods:The polysaccharides were prepared by water extraction and alcohol precipitation,deproteinization with trichloroacetic acid and decolorization with hydrogen peroxide.The physicochemical properties of polysaccharides were studied,and the possible structures were predicted by Ultraviolet-visible(UV-Vis)and infrared(IR)spectroscopy.Finally,the free radical scavenging ability was determined.Results:The polysaccharide from Z.jujuba cv.Banzao contained the content of total sugar and uronic acid with 52.30%and 46.22%,respectively,and showed 64.70%of esterification degree,which was an acidic pectin polysaccharide;UV-VIS and IR spectroscopy showed that the polysaccharide from Z.jujuba cv.Banzao had typical polysaccharide absorption peak,and contained no protein or nucleic acid.It was a pyranose withα-glycosidic bond,mainly composed of galacturonic acid,galactose,arabinose,glucose.The polysaccharides from Z.jujuba cv.Banzao displayed certain antioxidant activity and the free radical clearance of the polysaccharide at 1 mg/mL for DPPH radical,·OH and ABTS+·were 23.64%,21.01%and 13.84%,respectively.Conclusion:The polysaccharide from Z.jujuba cv.Banzao was an acidic pectin sugar mainly composed of galacturonic acid.Besides,it hadα-glycosidic bond and showed strong antioxidant capacity,which can be used as a potential antioxidant.
作者
李楠
张香飞
杨春杰
LI Nan;ZHANG Xiang-fei;YANG Chun-jie(Department of Life Science,Yuncheng University,Yuncheng,Shanxi 044000,China;Department of Mechanical and Electrical Engineering,Yuncheng University,Yuncheng,Shanxi 044000,China)
出处
《食品与机械》
北大核心
2022年第10期24-28,49,共6页
Food and Machinery
基金
山西省高等学校科技创新项目(编号:2020L0567)
山西省重点学科建设经费资助项目(编号:FSKSC)
运城学院院级科研项目(编号CY-2020023)。
关键词
板枣
多糖
理化性质
结构表征
抗氧化活性
Zizyphus jujuba cv.Banzao
polysaccharide
physicochemical properties
structure characterization
antioxidant activity
