摘要
报道了研究多糖酯化度(DE)的新方法,以金丝小枣为原料,从其水溶性提取物中分离得到金丝小枣粗多糖,经DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱层析,得到一个均一的金丝小枣多糖组分(ZSP3c)。红外傅立叶转换光谱(FT-IR)测定ZSP3c的DE为41.9%,与化学滴定法测定结果之间没有显著性差异(P>0.05),说明FT-IR测定多糖的DE是准确可行的。
A new method was applied for the study of the degree of esterification （DE） of polysaccharide.A crude polysaccharide was isolated from the watersoluble extracts of ZiZyphus Jujuba cv. Jinsixiaozao, and a watersoluble polysaccharide（ZSP3c） was purified by DEAE-SepharoseCL-6B, Sepharose CL-6B and Sephadex G- 200 column chromatography.The degree of esterification （DE） value of ZSP3c was 41.9% by fourier transform infrared spectrophotometer（FT-IR） ,no significant differences（ P 〉0.05 ） were found between the result of FT-IR and chemistry titration.The method was accurate and feasibility.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第6期343-345,共3页
Science and Technology of Food Industry
基金
江南大学食品学院青年博士科研创新开放基金(FS-200801)
食品科学与技术国家重点实验室资助
关键词
金丝小枣
多糖
分离纯化
酯化度
ZiZyphus Jujuba cv. Jinsixiaozao
polysaccharide
isolation and purification
degree of esterification
