摘要
采用硅胶柱色谱、MCI柱色谱、半制备高效液相色谱等技术从毛大丁草(Gerbera piloselloides)全草95%乙醇提取物中分离纯化得到10个化合物。根据其理化性质、波谱数据(UV、IR、MS、NMR)、圆二色谱(CD)和单晶X射线衍射等技术鉴定化合物结构,分别为3′R-毛大丁香豆素A(1a)、3′S-毛大丁香豆素A(1b)、gymnastone(2)、大丁苷(3)、divaricataester C(4)、木犀草素(5)、咖啡酸甲酯(6)、绿原酸乙酯(7)、6-(β-D-glucopyranosyloxy)-7-methoxy-5-benzoranpropanoic acid methyl ester(8)和glucozaluzanin C(9)。其中化合物1a和1b为新化合物且为光学对映异构体,通过手性拆分得到,并经量子化学计算ECD确定了其绝对构型。化合物1和1a/1b能显著提高LPS损伤后的大鼠小肠隐窝上皮细胞IEC-6的存活率。
Ten compounds were isolated from the 95%ethanol extract of the whole plant of Gerbera piloselloides by silica gel column chromatography,MCI column chromatography and semi-preparative HPLC methods.Their structures were identified on the basis of physicochemical properties,spectral data(UV,IR,MS and NMR),circular dichroism(CD)spectra and single crystal X-ray diffraction analysis as 3′R-gerpilosecoumarin A(1a),3′Sgerpilosecoumarin A(1b),gymnastone(2),gerberinside(3),divaricataester C(4),luteolin(5),caffeic acid methyl ester(6),ethyl chlorogenate(7),6-(β-D-glucopyranosyloxy)-7-methoxy-5-benzoranpropanoic acid methyl ester(8)and glucozaluzanin C(9).Among them,new compounds 1a and 1b were new compounds and optical enantiomers,which were obtained by chiral resolution,and their absolute configurations were determined by quantum chemical calculation ECD.Compounds 1 and 1a/1b significantly increased the survival of IEC-6 in rat small intestinal crypt epithelial cells after LPS injury.
作者
高哈
赵晨旭
罗心遥
李靖荣
孙彦君
张涛
邹忠梅
GAO Ha;ZHAO Chen-xu;LUO Xin-yao;LI Jing-rong;SUN Yan-jun;ZHANG Tao;ZOU Zhong-mei(Henan University of Chinese Medicine,Zhengzhou 450046,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union College,Beijing 100193,China;Pharmacy Faculty Hubei University of Chinese Medicine,Hubei Provincial Key Laboratory of Chinese Medicine Resource,Wuhan 430065,China;Guizhou Medical University,Guizhou 550000,China)
出处
《药学学报》
CAS
CSCD
北大核心
2022年第9期2774-2779,共6页
Acta Pharmaceutica Sinica
基金
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-028)
国家“重大新药创制”科技重大专项(2019ZX09735002).
