摘要
目的探讨Hedgehog信号通路、血清神经生长因子(NGF)、前列腺素(PGS)、骨钙素(BGLAP)在颅脑损伤后加速骨折愈合中的作用和相互关系。方法选取2017年1月至2020年1月在河北省邢台市第三医院就诊的138例因车祸导致的新鲜闭合性股骨干骨折患者,根据是否伴有颅脑损伤分为研究组(n=60)和对照组(n=78)。比较两组术后4、8、12周的骨痂面积和骨折愈合时间,采用ELISA检测术后1 d、术后1、4、8、12周血清NGF、BGALP、PGS、印度豪猪蛋白(Ihh)和锌指转录因子1(Gli1)水平。采用Pearson相关性分析探究上述各因素之间的相关性。结果研究组愈合时间明显短于对照组[(11.69±1.47)周vs.(14.02±1.83)周,P<0.05]。两组术后4、8、12周骨痂面积比较,研究组明显大于对照组(P<0.05)。术后1 d至术后12周,研究组血清Ihh、Gli1表达水平均明显高于对照组(P<0.05),血清NGF、PGS、BGLAP表达水平亦明显高于对照组(P<0.05)。Pearson相关性分析表明,血清NGF、BGLAP、PGS水平与骨痂面积呈正相关(P<0.05),血清Ihh、Gli1水平与血清NGF、BGLAP、PGS水平及骨痂面积也呈正相关(P<0.05)。结论颅脑损伤后Hedgehog信号通路相关蛋白上调,可能与NGF、PGS、BGLAP表达升高存在密切联系,该通路可能参与颅脑损伤后骨折加速愈合。
Objective To investigate the effect and mutual relation of Hedgehog signal pathway,serum nerve growth factor(NGF),prostaglandins(PGS),bone Gla protein(BGLAP)in accelerating fracture healing after craniocerebral injury.Methods A total of 138 patients with fresh closed femoral shaft fractures caused by car accidents treated in Xingtai Municipal Third Hospital from January 2017 to January 2020 were selected and divided into the research group(n=60)and control group(n=78)according to whether accompanying by craniocerebral injury.The fracture healing time and callus area at postoperative 4,8 and 12 weeks were compared between the two groups.The levels of serum NGF,BGLAP,PGS,Indian hedgehog(Ihh)and Gli family zinc finger protein 1(Gli1)at postoperative 1 day,1,4,8 and 12 weeks were detected by ELISA.The Pearson correlation analysis was used to analyze the correlation among the above factors.Results The healing time of the research group was significantly shorter than that of the control group[(11.69±1.47)weeks vs.(14.02±1.83)weeks,P<0.05].The callus area at postoperative 4,8 and 12 weeks in the research group were significantly larger than those in the control group(P<0.05).The levels of serum Ihh and Gli1 from postoperative 1 d to 12 weeks in the research group were significantly higher than those in the control group(P<0.05),and the expression levels of serum NGF,PGS,BGLAP in the research group were also significantly higher than those in the control group(P<0.05).The Pearson correlation analysis showed that serum NGF,BGLAP and PGS levels were positively correlated with the callus area(P<0.05),serum Ihh and Gli1 levels were also positively correlated with serum NGF,BGLAP and PGS levels and callus area(P<0.05).Conclusion The up-regulation of Hedgehog pathway related proteins after craniocerebral injury may be closely related to the increase of NGF,PGS and BGLAP expressions.This pathway may be involved in accelerating fracture healing after craniocerebral injury.
作者
李晖
李海冰
刘欣哲
张华
LI Hui;LI Haibing;LIU Xinzhe;ZHANG Hua(Department of Orthopedics,Xingtai Third Hospital,Xingtai,Hebei 054099,China;Department of Orthopedics,The Second Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China)
出处
《重庆医学》
CAS
2022年第19期3268-3272,共5页
Chongqing medicine
基金
河北省卫生健康委员会2021年度医学科学研究课题项目(20210158)。
