摘要
目的:通过观察龟鹿二仙胶及其拆方对豚鼠软骨细胞Ⅱ型胶原和蛋白多糖合成的影响,探讨龟鹿二仙胶的配伍机制。方法:体外培养豚鼠膝关节软骨细胞,免疫组化染色检测龟鹿二仙胶及其拆方对软骨细胞Ⅱ型胶原合成的影响,Alcian Blue染色检测软骨细胞蛋白多糖合成。结果:龟鹿二仙胶促进软骨细胞Ⅱ型胶原和蛋白多糖表达极显著(P<0.01),龟板和鹿角均能显著提高Ⅱ型胶原和蛋白多糖表达(P<0.05),但药效显著低于龟鹿二仙胶(P<0.05);人参和枸杞对Ⅱ型胶原和蛋白多糖表达均无明显促进作用。结论:龟鹿二仙胶可显著促进软骨细胞Ⅱ型胶原和蛋白多糖的表达,这是其保护软骨细胞,延缓软骨退变的作用机制之一。龟鹿二仙胶发挥此作用的主要药物是龟板和鹿角;人参和枸杞本身并不能促进Ⅱ型胶原和蛋白多糖表达,而与龟板和鹿角配合,可明显增强龟板和鹿角的促进软骨细胞Ⅱ型胶原和蛋白多糖表达的作用。
Objective： To investigate the mechanism of Guilu Erxian Glue compatibility by detecting the effect of Collagen type Ⅱ and proteoglycan of guinea pig＇s Chondrocyte.Method： Extract chondrocyte from rat＇s knee and cultured in vitro.Detecting collagen type Ⅱ by immunohistochemistry stain and proteoglycan by Alcian Blue stain.Result： GuiLu ErXian Glue can highly significantly enhance the expression of collagen type Ⅱ and proteoglycan（P0.01）.Carapax testudinis and antler can also significantly enhance the expression of collagen type Ⅱ and proteoglycan（P0.05）,but is not better than Guilu Erxian Glue（P0.05）.Ginseng and Lycium has no obviously effect in the expression of collagen type Ⅱ and proteoglycan.Conclusion： One of the main mechanism that Guilu Erxian Glue can protect cartilage from ageing is that it can highly significantly enhance the expression of collagen type Ⅱ and proteoglycan secreted by rat＇s chondrocyte.The main active substance of Guilu Erxian Glue are Carapax testudinis and antler.Ginseng and Lycium both can not enhance the expression of collagen type Ⅱ and proteoglycan,but they can help Carapax testudinis and antler enhance the expression of collagen type Ⅱ and proteoglycan.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第11期2524-2528,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.30772814)
福建省自然科学基金(No.2007J0098
No.C0710028)
福建省高等学校新世纪优秀人才支持计划(No.闽教科[2007]20号)~~
关键词
龟鹿二仙胶
豚鼠
软骨细胞
Ⅱ型胶原
蛋白多糖
Guilu Erxian Glue
Guinea pig＇s
Chondrocyte
Collagen type Ⅱ
Proteoglycan
