摘要
近年来,异体皮来源极度匮乏,给大面积危重烧伤患者的救治带来了极大挑战。而异种猪皮虽然结构功能与人皮肤相似,但受免疫排斥反应、猪内源性反转录病毒感染等因素影响,其临床应用受到限制。随着基因编辑技术的发展,特别是成簇规律间隔短回文重复序列(CRISPR)/CRISPR相关蛋白9系统的出现,使一次实施多位点靶基因编辑成为可能,为异种猪皮治疗烧伤创面带来了广阔的应用前景。该文着重对异种猪皮移植治疗临床烧伤创面的进展、存在问题、基因修饰/编辑策略及其应用研究进行讨论。
In the recent years,the shortage of allo-skin sources has resulted in great challenges for salvage of patients with large area severe burns.Although being similar to human skin in construction and function,the clinical application of xenogenic porcine skin in burn wound management is limited due to factors including immuno-rejection,porcine endogenous retroviruses infection,etc.With the development of gene editing technology,especially the emerge of clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated protein-9 system,multiple target genes could be possibly edited at the same time,which will bring broad prospect for the application of xenogenic porcine skin in the treatment of burn wounds.The paper mainly discusses the development,the existed barrier,the strategies of gene modification/editing,and the applications and research of xenogenic porcine skin xenografts in the clinical treatment of burn wound.
作者
段红杰
Duan Hongjie(Burn Institute of Department of Burns and Plastic Surgery,the Fourth Medical Center of PLA General Hospital,Beijing 100048,China)
出处
《中华烧伤与创面修复杂志》
CAS
CSCD
北大核心
2022年第9期805-809,共5页
Chinese Journal of Burns And Wounds
基金
军队后勤科研重大项目(ALB19J001)。
关键词
烧伤
生物敷料
移植
异种
基因编辑
猪皮
创面修复
Burns
Biological dressings
Transplantation,heterologous
Gene editing
Porcine skin
Wound repair
