摘要
以榉树(Zelkova serrata Thunb)为试验对象,选择苗高约10 cm、地径约1 mm、长势一致的榉树幼苗为供试幼苗。2017年6月份在南京林业大学下蜀林场温室进行盆栽试验,分别接种摩西球囊霉(Glomus mosseae)、扭形球囊霉(Glomus tortuosum),以不接种丛枝菌根真菌(AMF)为对照(CK),每种处理24盆,每盆基质约2.5 kg(以下蜀林场林地5~30 cm的表层土、黄沙、蛭石,等体积混合作为盆栽基质);接菌后温室培育三个月为丛枝菌根真菌的侵染期,后培育一年为植物生长期。接菌后温室培养3个月,测定菌根真菌侵染率后,进行盐胁迫;每种菌处理分别设置4个浓度盐处理(0、50、100、150 mmol·L^(-1),各12个重复,共进行2个月,期间定期补充试验设计的在原“霍格兰营养液(缺磷、不含硝酸钙)”基础上补加磷质量分数为25%的改进营养液。2018年9月份,每种处理选取3株长势一致的幼苗,测定侵染率、根系形态、根系氮磷养分及酶活性;采集根际土,测定全磷、全氮、有效磷、碱解氮;分析盐和丛枝菌根真菌协同胁迫对榉树根系的影响。结果表明:盐胁迫处理,降低了摩西球囊霉、扭形球囊霉处理的侵染率。当盐浓度达到100 mmol·L^(-1)时,榉树根表面积、根长度、根尖数显著降低;而接种摩西球囊霉,增加了榉树根表面积、根长度、根尖数。盐胁迫处理时,榉树根系磷质量分数显著降低;接种摩西球囊霉,则显著提高了根系磷质量分数。随盐浓度增高,摩西球囊霉处理的硝酸还原酶活性显著提高,摩西球囊霉处理显著增强了烟酰胺腺嘌呤二核苷酸氧化酶活性。随着盐浓度提升,根际土壤碱解氮质量分数呈上升趋势,其中盐浓度为150 mmol·L^(-1)时,摩西球囊霉处理比对照处理显著降低了土壤碱解氮质量分数。盐胁迫处理会抑制植物根系生长;接种丛枝菌根真菌可以促进植物根系生长,改善植物根际土壤环境、加强养分吸收、调�
Zelkova serrata Thunb was used as the test subject,and beech seedlings of approximately 10 cm in height,1 mm in ground diameter and uniform growth were selected as the test seedlings.Pot trials were conducted in June 2017 in the greenhouse of Nanjing Forestry University,Xiashu Forestry Field.Inoculation with Glomus mosseae and Glomus tortuosum,respectively,with no inoculation of arbuscular mycorrhizal fungi(AMF)as the control(CK).24 pots of each treatment,with about 2.5 kg of substrate per pot(topsoil,yellow sand,and vermiculite of 5-30 cm from the forest floor of the Xiashu forestry,mixed in equal volume as potting substrate).After inoculation,the plants were cultivated in the greenhouse for three months as the infestation period of arbuscular mycorrhizal fungi,and then cultivated for 1 a as the plant growth period.After 3-month greenhouse incubation after inoculation,the rate of mycorrhizal fungal infestation was measured and salt stress was applied.Four concentration salt treatments(0,50,100,150 mmol·L^(-1))were set up for each bacterial treatment,with 12 replicates each,for a total of 2 months.During this period,the Hogeland nutrient solution(phosphorus deficient,without calcium nitrate)with a phosphorus mass fraction of 25%was supplemented regularly.In September 2018,three seedlings of uniform growth were selected from each treatment to determine the infestation rate,root morphology,root nitrogen and phosphorus nutrients and enzyme activity.Rhizosphere soils were collected to determine total phosphorus,total nitrogen,effective phosphorus,and alkali-hydrolyzable nitrogen.The effects of salt stress and inoculation of arbuscular mycorrhizal fungi were analyzed on the root system of Z.serrata.The salt stress treatment reduced the infestation rate of inoculation by Glomus mosseae and G.tortuosum treatments.When the salt concentration reached 100 mmol·L^(-1),the root surface area,root length,and root tip number of Z.serrata were significantly reduced,while inoculation with G.mosseae increased the root surface ar
作者
曾婧祎
朱凌骏
马仕林
张金池
王金平
刘京
聂晖
陈美玲
Zeng Jingyi;Zhu Lingjun;Ma Shilin;Zhang Jinchi;Liu Jing;Nie Hui;Chen Meiling(Nanjing Forestry University,Nanjing 210037,P.R.China;Guodian Environmental Protection Research Institute Co.,Ltd.;Nanjing Forestry University)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2022年第9期11-17,共7页
Journal of Northeast Forestry University
基金
江苏省科技计划项目(BE2022420)
江苏省林业科技创新与推广项目(LYKJ[2021]30)
百山祖国家公园科学研究项目(2021ZDLY01)
江苏高校优势学科建设工程项目(PAPD)。
关键词
盐胁迫
丛枝菌根真菌
榉树
根系
根际土壤
Salt stress
Arbuscular mycorrhizal fungi
Zelkova serrata
Root system
Rhizosphere soil
