摘要
目的建立测定大鼠血浆中红景天苷浓度的高效液相色谱串联质谱(HPLC-MS/MS)方法,用于苗药金骨莲给药后大鼠体内红景天苷的药代动力学评价。方法雄性SD大鼠灌胃给药前及给药后不同时间点取血,血浆采用蛋白沉淀法进行处理,以水杨苷为内标,采用HPLC-MS/MS法测定血浆中红景天苷的浓度,并用MaS Studio软件计算药代动力学参数。结果血浆中红景天苷在10~1000 ng/mL范围内线性关系良好(r=0.9991),最低定量下限为10 ng/mL,批内、批间精密度RSD为1.9%~6.4%,准确度为100.1%~108.0%,基质效应为32.1%~61.9%(RSD为11.0%~14.1%),提取回收率为33.3%~51.7%(RSD为11.4%~11.7%),稳定性RSD为1.4%~9.6%,准确度为97.0%~107.3%。第1、14日给药金骨莲后红景天苷的t_(1/2)分别为(2.20±0.48)h、(0.92±0.12)h,t_(max)分别为(0.80±0.30)h、(0.67±0.00)h,C_(max)分别为(1198.73±163.84)ng/mL、(1123.57±88.35)ng/mL,AUC_(0~t)分别为(2946.02±590.02)ng·h/mL、(2861.62±514.98)ng·h/mL。结论本研究建立的方法适用于金骨莲给药后大鼠体内红景天苷的药代动力学研究。给药后红景天苷迅速被吸收并到达峰浓度,连续给药14 d未发现体内蓄积作用。
Objective To establish a high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)method for the determination of salidroside in plasma of rats;To evaluate the pharmacokinetics of salidroside in rats after administration of Jingulian Capsules.Methods Blood was collected from male SD rats at different time points before and after administration.The plasma was processed by protein precipitation method,and salicin was used as the internal standard.The concentration of salidroside in plasma was determined by HPLC-MS/MS method,and the pharmacokinetic parameters were calculated with MaS Studio software.Results The standard curve of salidroside in plasma was linear(r=0.9991)over the concentration ranges of 10-1000 ng/mL.The lower limits of quantification was 10 ng/mL.The relative errors and coefficients of variation for intra-and inter-assay at four quality control levels were 1.9%-6.4%,and 100.1%-108.0%,respectively.The matrix effect was 32.1%-61.9%with RSD from 11.0%to 14.1%.The extraction recovery rate was 33.3%-51.7%with RSD from 11.4%to 11.7%.The RSD of stability was 1.4%-9.6%and accuracy was 97.0%-107.3%,respectively.The t_(1/2),t_(max),C_(max) and AUC_(0/t) of salidroside after administration of Jingulian Capsules at first and fourteenth day were(2.20±0.48)h and(0.92±0.12)h,(0.80±0.30)h and(0.67±0.00)h,(1198.73±163.84)ng/mL and(1123.57±88.35)ng/mL,(2946.02±590.02)ng·h/mL and(2861.62±514.98)ng·h/mL,respectively.Conclusion The method established in this study is suitable for the pharmacokinetic study of salidroside in rats after administration of Jingulian Capsules.The salidroside can be quickly absorbed and reach the peak concentration,and no accumulation is observed for 14 days administration in rats.
作者
陈腾飞
练东银
高云航
宋玲
李晗
侯红平
李建良
彭博
叶祖光
张广平
CHEN Tengfei;LIAN Dongyin;GAO Yunhang;SONG Ling;LI Han;HOU Hongping;LI Jianliang;PENG Bo;YE Zuguang;ZHANG Guangping(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100029,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2022年第9期106-110,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家重点研发计划(2018YFC1708100、2018YFC1708105)。
