摘要
用分子印迹和沉淀聚合技术,以石杉碱甲(Hup A)为模板、α-甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸乙酯为交联剂成功制备了分子印迹聚合物(MIP)。采用FTIR、SEM、TGA对MIP进行了表征。通过静态吸附、动态吸附、体外释药和3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴盐(MTT)法对MIP性能进行了评价。结果表明,MIP对Hup A存在特异性和非特异性两种结合位点。市售Hup A片在1 h内快速释放,累积释放率达90%以上,有明显的突释现象。而MIP在1 h后释放平缓,在24 h附近达到平衡,可以达到缓慢释放的效果。释放动力学符合Peppas模型,释放指数为0.48,为非Fickian扩散机理。MIP生物相容性良好,L929小鼠成纤维细胞对MIP的细胞毒性数据显示,细胞存活率>93%。
Molecularly imprinted polymer(MIP)was prepared by molecular imprinting and precipitation polymerization using huperzine A(Hup A)as template,α-methacrylic acid(MAA)as functional monomer,and ethylene glycol dimethyl acrylate as crosslinking agent.The synthesized MIP were then characterized by FTIR,SEM and TGA,followed by property evaluation by static adsorption,dynamic adsorption,in vitro drug release and 3-(4,5-dimethyl-2-thiazolyl)-2,5-di-phenyltetrazolium bromide(MTT)assay.The results showed that Hup A was polymerized in MIP through specific and nonspecific binding.The commercially available Hup A tablets was rapidly released within 1 h showing cumulative release rate of more than 90%with obvious sudden release phenomenon,while MIP showed sustained release of Hup A after the first hour and reached equilibrium about 24 h,demonstrating slow release effect.The release kinetics conformed to the Peppas model with a release index of 0.48,implying a non Fickian diffusion mechanism.Moreover,in vitro cytotoxicity assay indicated that the survival rate of L929 mouse fibroblasts was higher than 93%when treated with MIP,which suggested good biocompatibility.
作者
贾朝
温惠云
黄赛朋
郭鹏琦
曹波
陈欣
王雅茹
薛伟明
JIA Zhao;WEN Huiyun;HUANG Saipeng;GUO Pengqi;CAO Bo;CHEN Xin;WANG Yaru;XUE Weiming(School of Chemical Engineering,Northwest University,Xi′an 710069,Shaanxi,China;Shangluo University,Shangluo 726000,Shaanxi,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2022年第8期1612-1618,共7页
Fine Chemicals
基金
陕西省自然科学基金(2018JM2037,2020JQ-609)
陕西省教育厅项目(20JK0610)
陕西省重点研发计划(2020GY-313)
中国博士后科学基金(2020M683701XB)。
关键词
石杉碱甲
分子印迹
沉淀聚合法
药物缓释
功能材料
huperzine A
molecular imprinting
precipitation polymerization
drug delivery
functional materials
