摘要
目的探讨黄精多糖(PSP)对模拟航天狭小空间诱导的小鼠认知功能损伤模型的改善作用及可能的分子机制。方法将72只雄性ICR小鼠按体重随机分成空白组、模型组、阳性药组(石杉碱甲)、黄精多糖(100、200、400 mg/kg)组;除空白组外,其它组别小鼠进行慢性束缚35 d以建立模拟航天狭小空间诱导的认知障碍模型,同时进行体重监测(束缚强度10 h/d,22:00~次日8:00);随后依次进行空场实验、物体认知实验、Morris水迷宫、避暗等行为学检测实验,取血清、海马并测定小鼠血清和海马组织内超氧化物歧化酶(SOD)、丙二醛(MDA)水平,海马组织内氧化氢酶(CAT)水平、海马皮质酮(CORT)及神经递质乙酰胆碱(Ach)含量。结果空场实验结果表明慢性束缚造模不影响小鼠自主活动能力;认知行为学检测表明,与空白组相比,模型组小鼠在物体认知实验中的相对辨别指数显著降低(P<0.01,P<0.001),在避暗实验中的入暗潜伏期显著缩短、错误次数显著增加(P<0.05);水迷宫寻台潜伏期显著延长,穿台次数显著降低(P<0.05);血清和海马中CAT显著降低、MDA显著增加(P<0.05,P<0.001),海马组织内SOD显著降低,CORT、Ach含量显著升高(P<0.05)。与模型组相比,黄精多糖各给药组灌胃给药后均能显著改善上述指标。结论黄精多糖可改善模拟航天狭小空间所致小鼠认知障碍,其作用机制可能与改善血清海马氧化应激、调节海马组织内神经递质水平以及HPA轴相关,本研究发现为黄精多糖用于航天应激损伤防护药物的研发奠定理论基础。
Objective To study the protective effect and mechanism of action of Polygonati rhizoma polysaccharide(PSP)on the cognition deficits induced by simulating an enclosed space in mice.Methods Male ICR mice(n=72)were randomly divided into four groups:control group,model group,positive control drug treatment group(huperzine-A),and PSP treatment group(100,200,400 mg/kg).With the exception of the control group,all other mice were subjected to chronic restraint stress(CRS)for 35 days to establish a mouse model of cognitive impairment(restraint intensity 10 h/d,22:00 until 8:00 the next day)and body weight monitoring was conducted.Then,behavioral tests were performed,including an open field test,object recognition experiment,Morris water maze task,and a passive avoidance method.The levels of Superoxide dismutase(SOD)and Malondialdehyde(MDA)in the serum and the hippocampus,and the levels of Catalase(CAT),Corticosterone(CORT),and Acetylcholine(Ach)in the hippocampus were measured.Results The open field test showed that CRS did not affect the locomotor activities of any of the groups.Compared with the control group,the discrimination index(DI)of the CRS model group in the object recognition experiment was significantly decreased(P<0.01,P<0.001),the errors times increased and the latent period decreased in the passive avoidance test(P<0.05),and a longer escape latency in MWM(Morris water maze)was observed in the CRS model group(P<0.05).Furthermore,in the CRS model group,the SOD and CAT levels were significantly decreased,MDA was increased,and CORT and Ach were significantly increased both in the serum and hippocampus(P<0.05,P<0.001).In comparison with the CRS model group,the above indicators were markedly reversed in the PSP groups.Conclusions The result suggested that PSP treatment can improve the cognitive impairment induced by simulating an enclosed space in mice,by mechanisms that involve the amelioration of oxidative stress damage,elevation of neurotransmitter levels,and regulation of the hypothalamic-pituitary-adrenal ax
作者
刘雨培
张瑛毓
范蓓
刘新民
陈颖
卢聪
王凤忠
LIU Yupei;ZHANG Yingyu;FAN Bei;LIU Xinmin;CHEN Ying;LU Cong;WANG Fengzhong(Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100013)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2022年第4期494-503,共10页
Acta Laboratorium Animalis Scientia Sinica
基金
中国农业科学院基本科研业务费专项院级统筹项目(Y2022LM09)
中国农业科学院创新工程所级重点任务-安神助眠类功能性产品精准设计制造(S2020JBKY-20)
中国农业科学院农产品加工研究所创新工程(CAAS-ASTIP-2022-IFST)。
关键词
黄精多糖
航天应激
慢性束缚应激
认知障碍
机制研究
Polygonati rhizoma polysaccharide
aerospace stress
chronic restraint stress(CRS)
cognitive impairment
mechanism
