摘要
为研究多花黄精种质资源品质性状与矿质元素的遗传多样性并筛选优质种质,本试验以收集自国内的43份多花黄精种质为材料,测定其7个品质性状与15种矿质元素含量并进行多样性分析、相关性分析、主成分分析、聚类分析及隶属函数法综合评价。结果表明,43份种质多糖、总灰分、醇溶性浸出物、水分、部分重金属及有害元素含量均符合2020版《中华人民共和国药典》的标准;品质性状的变异系数在10.4%~46.7%之间,遗传多样性指数在1.600~2.015之间;矿质元素含量的变异系数在23.6%~92.7%之间,遗传多样性指数在1.526~2.006之间。相关性分析显示,品质性状间、矿质元素间、品质性状与矿质元素间共76对指标之间达到显著(P<0.05)或极显著(P<0.01)相关水平,矿质元素之间相关关系复杂但整体协同作用较强,矿质元素对品质性状则抑制作用较强。主成分分析将11种矿质元素简化为3个主成分,累计方差贡献率为66.71%,筛选出K、Zn、Fe、P作为评价多花黄精种质资源的特征元素。系统聚类分析在距离为100时将43份多花黄精种质资源分为4个类群,隶属函数法综合评价与聚类分析的结果基本一致,其中排名前十的优质种质为S33、S27、S29、S28、S31、S10、S17、S36、S32、S20。综上表明,多花黄精种质的品质性状与矿质元素含量变异明显,具有丰富的遗传多样性。本结论可为优质多花黄精种质选育提供理论参考和依据。
In order to study the genetic diversity of quality traits and mineral elements of Polygonatum cyrtonema Hua germplasm resources and screen high-quality germplasm,43 P.cyrtonema Hua germplasm resources in China were collected,and the contents of 7 quality traits and 15 mineral elements were determined and counted.The comprehensive evaluation including diversity analysis,correlation analysis,principal component analysis,clustering analysis and membership function method was conducted.The results showed that the contents of polysaccharide,total ash,alcohol-soluble extract,water,some heavy metals and harmful elements in reference germplasm resources met the standards of China Pharmacopoeia(2020 edition).The coefficient of variation of quality traits was 10.4%to 46.7%,and the genetic diversity index was 1.600 to 2.015.The coefficient of variation of mineral elements was 23.6%to 92.7%,and the genetic diversity index was 1.526 to 2.006.Correlation analysis showed that significant levels(P<0.05)or extremely significant levels(P<0.01)were reached among a total of 76 pairs of indicators.The correlation between mineral elements was complex but body synergy was strong,and the inhibition effect of mineral elements on quality traits was strong.Eleven mineral elements were simplified into three principal components by principal component analysis,with the contribution rate of cumulative variance of 66.71%,and K,Zn,Fe and P were screened out as the characteristic elements for evaluating the germplasm resources of P.cyrtonema Hua.When the distance was 100 by systematic clustering analysis,43 germplasm resources of P.cyrtonema Hua were divided into four groups.The results of comprehensive evaluation by membership function method were basically consistent with the results of clustering analysis,and the top ten high-quality provenances were S33,S27,S29,S28,S31,S10,S17,S36,S32,and S20,respectively.The variation of quality traits and mineral element germplasm resources of P.cyrtonema Hua was significant,and there was rich genetic dive
作者
王灿彬
潘克琴
王华磊
罗春丽
李金玲
陈松树
李丹丹
梁瑾
刘红昌
罗夫来
黄明进
Wang Canbin;Pan Keqin;Wang Hualei;Luo Chunli;Li Jinling;Chen Songshu;Li Dandan;Liang Jin;Liu Hongchang;Luo Fulai;Huang Mingjin(College of Agriculture,Guizhou University/Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guizhou 550025,China)
出处
《山东农业科学》
北大核心
2024年第6期55-64,共10页
Shandong Agricultural Sciences
基金
国家重点研发计划项目“林下生态种植黄精、淫羊藿等中药材产业关键技术研究与应用示范”(2021YFD1601002)
黄精种质资源收集保存鉴定评价(黔农计财[2022]10号)
贵州省药用植物繁育与种植人才基地项目(黔人领发[2020]2号)
贵州特色药用植物种苗繁育工程技术研究中心项目(黔科合平台人才[2020]2101号)
基于DNA分子标记的黄精遗传多样性研究及优异种质资源筛选(筑科合同[2022]3-3号)
贵州省中药材现代农业产业技术体系项目(GZCYTX2024)
道地药材黄精高端产品研发及产业化应用研究(铜科成合[2020]02号)。
关键词
多花黄精
种质资源
品质性状
矿质元素
遗传多样性
综合评价
Polygonatum cyrtonema Hua
Germplasm resources
Quality traits
Mineral elements
Genetic diversity
Comprehensive assessment
