摘要
木姜子是我国南方特有的香料植物。为更好的开发木姜子资源,使用水蒸气蒸馏法(SD)提取木姜子果实的挥发油(LPO),通过气相色谱-质谱联用法(GC-MS)进行成分分析,并采用抑菌圈法测定其对果生刺盘孢菌和尖孢镰刀菌的抑菌效果。结果表明:SD法提取木姜子挥发油的得率为1.6%,共鉴定出19个成分,占挥发油总量的98.8%。其中含量最高的是柠檬醛(30.10%)、柠檬烯(29.20%)、芳樟醇(6.45)和桉叶素(5.65%)等单萜烯类和单萜类物质。LPO对尖孢镰刀菌的抑菌活性优于果生刺盘孢菌,20 mg·mL时菌丝生长抑制率均为100%。可见木姜子挥发油富含萜类物质,有良好的抑菌活性,具有作为生物农药开发的潜力。
Litsea pungens Hemsl.is a unique spice plant in South China.In order to better exploit Litsea pungens resources,the essential oil from the fruit of Litsea pungens(LPO)was extracted by steam distillation(SD)method,and the components were analyzed by gas chromatography-mass spectrometry(GC-MS).The antifungal effect of LPO on Colletotrichumz fructicola and Fusarium oxysporum was determined by inhibition zone method.The results showed that the yield of essential oil extracted by SD method was 1.6%,and 19 components were identified,accounting for 98.8%of the total essential oil.Among them,citral(30.10%),limonene(29.20%),linalool(6.45%)and eucalyptus(5.65%)were the monoterpenes and monoterpenes with the highest content.The antifungal activity of LPO against F.oxysporum was better than that of C.fructicola,and the inhibition rate of mycelium growth was 100%at 20 mg·mL.In conclusion,the LPO is rich in terpenoids,has good antifungal activity,and has the potential to be developed as a biological pesticide.
作者
陈彦宏
张鑫瑶
陈旺
肖汉永
周莉君
CHEN Yanhong;ZHANG Xinyao;CHEN Wang;XIAO Hanyong;ZHOU Lijun(College of Life Science,Sichuan Agricultural University,Ya’an 625000,China)
出处
《四川林业科技》
2022年第4期124-128,共5页
Journal of Sichuan Forestry Science and Technology
基金
四川省大学生创新创业训练项目(S202010626108)。
关键词
木姜子
挥发油
抑菌活性
Litsea pungens
Essential oil
Antifungal activity
