摘要
乳杆菌溶源性噬菌体在乳品发酵工业中分布广泛,其侵染特性特殊,具有一定的隐蔽性,但在一定条件下,可能被诱导转变为烈性噬菌体,使发酵剂菌株裂解,导致发酵失败。因此,溶源性噬菌体已成为发酵生产过程中的主要安全隐患之一。该研究以发酵乳杆菌为研究对象,利用不同诱导方式对其诱导,并评价热处理及不同化学杀菌剂对诱导出噬菌体的灭活效果。结果显示,发酵乳杆菌(Lactobacillus fermentum)IMAU32579经1.9μg/mL的丝裂霉素C诱导后,成功获得噬菌体,将其命名为LFP-VB1。形态学分析显示,该噬菌体属于长尾噬菌体科。该噬菌体对热敏感,63℃处理4 min即完全失活。化学杀菌剂对其灭活效果有限,800 mg/L NaClO溶液处理60 min仅使其下降6.29个对数级。
Lysogenic phages of Lactobacillus spp.are widely distributed in dairy fermentation setting.Their special infection characteristics are somewhat hidden and arduous to detect.However,under certain conditions,these phages could cause lysis of fermenting lactic acid bacteria strains,and eventually lead to fermentation failure.Therefore,lysogenic phages have become one of the major safety hazards in dairy fermentation.The Lactobacillus fermentum IMAU32579 was induced and the inactivation effects of heat treatment and different chemical biocides were evaluated.The results showed that a bacteriophage named as LFP-VB1 was successful induced from L.fermentum IMAU32579 after treatment with 1.9μg/mL mitomycin C.Morphology analysis revealed that this bacteriophage strain belonged to the family of Siphoviridae.It was observed that bacteriophage LFP-VB1 was sensitive to heat treatment(completely inactivated 63℃for 4 min).The inactivation effects of biocides were insignificant,6.29 lg decrease was observed after treatment with 800 mg/L NaClO for 60 min.This study can provide some theoretical basis for the constructing of phage control measures.
作者
郭蛇
许铭
张灿
朱含芳
陈霞
GUO She;XU Ming;ZHANG Can;ZHU Hanfang;CHEN Xia(Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education,Key Laboratory of Dairy Processing of inistry of Agriculture and Rural Areas,Key Laboratory of Dairy Biotechnology and Engineering of Inner Mongolia Autonomous Region,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第17期87-92,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(32160550,31760447,31301517)
中央引导地方项目(2021ZY0022)
内蒙古自然科学基金项目(2021MS03014,2019BS03003)。
关键词
发酵乳杆菌
溶源性噬菌体
诱导
灭活
化学消毒剂
热处理
Lactobacillus fermentum
lysogenic phage
induction
inactivation
chemical biocide
heat treatment
