摘要
在食品工业中,乳制品发酵失败的原因通常是由于噬菌体的侵染。在确保有效杀菌消毒的前提下,将不同化学消毒剂进行复配,使各杀菌剂性能达到互补,从而起到更好的增效作用,可有效避免噬菌体对发酵过程的侵染。本实验以植物乳杆菌IMAU10120及噬菌体P1、P2为研究对象,评价质量分数为0.3%过氧乙酸溶液与不同浓度次氯酸钠复合处理对其的灭活效果。实验结果表明:依次使用800 mg/kg次氯酸钠和0.3%过氧乙酸处理植物乳杆菌5 min后,植物乳杆菌的存活率为0.38%;0.3%过氧乙酸与800 mg/kg次氯酸钠直接混合可使噬菌体P1在10 min内完全失活;依次使用800 mg/kg次氯酸钠和过氧乙酸处理5 min后,可噬菌体P2下降5.05个对数级。本研究可为准确评价常用化学消毒剂对噬菌体的灭活效果提供一定的数据支持。
In food industry,the failure of dairy fermentation is usually caused by phage infection.On the premise of ensuring effective sterilization and disinfection,different chemical disinfectants are combined to achieve complementary and better synergistic inactivating effect,which can effectively avoid phage infection in the fermentation process.In this study,Lactobacillus plantarum IMAU10120 and bacteriophage P1,P2 were used as researching objects,the inactivate effects of 0.3%peracetic acid combined with sodium hypochlorite were evaluated.The results showed that treatment of 800 mg/kg sodium hypochlorite for 5 minutes followed by 0.3%peroxyacetic acid for 5 minutes,the survival rate of Lactobacillus plantarum was 0.38%.Phage P1 can be totally inactivated in 10 min when treated by 0.3%peracetic acid and 800 mg/kg sodium hypochlorite.For phage P2,treatment of 800 mg/kg sodium hypochlorite for 5 minutes followed by 0.3%peroxyacetic acid for 5 minutes can cause 5.05 log reduction.This study can provide some data support for accurately evaluating the inactivation effect of commonly used chemical disinfectants on bacteriophages.
作者
郭蛇
李伟娜
马瑞瑞
朱含芳
陈霞
GUO She;LI Weina;MA Ruirui;ZHU Hanfang;CHEN Xia(Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education,Key Laboratory of Dairy Processing of Ministry of Agriculture and Rural Areas,Inner Mongolia Agricultural University,Key Laboratory of Dairy Biotechnology and Engineering of Inner Mongolia Autonomous Region,Hohhot 010018,China)
出处
《中国乳品工业》
CAS
北大核心
2022年第5期20-23,共4页
China Dairy Industry
基金
国家自然科学基金项目(31760447)
内蒙古自治区自然科学基金项目(2021MS03014)。
关键词
植物乳杆菌噬菌体
过氧乙酸
次氯酸钠
复合处理
灭活
Lactobacillus plantarum phage
peracetic acid
sodium hypochlorite combined treatment
inactivation
