摘要
目的研究细胞色素酶2A6(CYP2A6)、乙醛脱氢酶(ALDH2)、FOS样抗原1(FOSL1)基因多态性对紫杉烷类基础化疗胃癌患者疗效的影响。方法选取2018年5月至2020年4月于该院接受治疗的胃癌患者152例为研究对象,均接受紫杉烷类基础化疗,使用聚合酶链反应(PCR)扩增法分析患者的CYP2A6、ALDH2、FOSL1基因多态性。采用Cox比例风险模型评估各影响因素对化疗效果的影响,采用Log-rank检验比较不同基因型的生存情况。结果CYP2A6基因有3个位点存在基因多态性,rs28399433(TT型96例、TG型48例、GG型8例)、rs8192720(CC型110例、CT型38例、TT型4例)、rs8192725(CC型105例、CT型42例、TT型5例)。ALDH2基因rs671位点GG型124例、GA型18例、AA型10例。FOSL1基因有2个位点存在基因多态性,rs1892901(GG型94例、GA型40例、AA型18例)、rs637571(GG型78例、GA型62例、AA型12例)。152例患者中化疗有效组86例,化疗无效组66例,两组患者的TNM分期、CYP2A6 rs8192725、ALDH2 rs671、FOSL1 rs1892901基因型比较,差异有统计学意义(P<0.05)。TNM分期(HR值为2.654)、CYP2A6 rs8192725基因型(HR值为2.085)、ALDH2 rs671基因型(HR值为2.960)以及FOSL1 rs1892901基因型(HR值为1.823)影响化疗结局(P<0.05)。化疗结束后随访1年,死亡36例。CYP2A6 rs8192725位点CC基因型、ALDH2 rs671位点GG基因型、FOSL1 rs1892901位点GG基因型的病死率均低于对应位点其他基因型,差异有统计学意义(P<0.05)。结论胃癌患者CYP2A6、ALDH2、FOSL1基因多态性可影响紫杉烷类基础化疗的疗效以及生存率,提示可根据患者基因型来指导个体化治疗。
Objective To study the effect of cytochrome enzyme 2A6(CYP2A6),aldehyde dehydrogenase(ALDH2),FOS like antigen 1(FOSL1)gene polymorphisms on the efficacy of taxane-based chemotherapy in patients with gastric cancer.Methods A total of 152 patients with gastric cancer who were treated as research objects in the hospital from May 2018 to April 2020 were selected and all received taxane-based basic chemotherapy.Polymerase chain reaction(PCR)amplification method was used to analyze the CYP2A6,ALDH2 and FOSL1 gene polymorphisms of the patients.The Cox proportional hazard model was used to evaluate the influence of various influencing factors on the efficacy of chemotherapy,and the Log-rank test was used to compare the survival status of different genotypes.Results The CYP2A6 gene had 3 genetic polymorphisms,namely rs28399433(96 cases of TT type,48 cases of TG type,8 cases of GG type),rs8192720(110 cases of CC type,38 cases of CT type,4 cases of TT type),rs8192725(105 cases of CC type,42 cases of CT type,5 cases of TT type).There were 124 cases of GG type,18 cases of GA type,and 10 cases of AA type at rs671 of ALDH2 gene.There were 2 genetic polymorphisms in the FOSL1 gene,namely rs1892901(94 cases of GG type,40 cases of GA type,18 cases of type AA),rs637571(78 cases of type GG,62 cases of type GA,and 12 cases of type AA).Among the 152 patients,86 cases were in the chemotherapy-effective group and 66 cases were in the chemotherapy-ineffective group.There were statistically significant differences on the TNM staging,CYP2A6 rs8192725,ALDH2 rs671,and FOSL1 rs1892901 genotypes between the two groups(P<0.05).TNM staging(HR=2.654),CYP2A6 rs8192725 genotype(HR=2.085),ALDH2 rs671 genotype(HR=2.960)and FOSL1 rs1892901 genotype(HR=1.823)affected the outcome of chemotherapy(P<0.05).A total of 36 patients died after 1 year follow-up.The mortality of CC genotype at CYP2A6 rs8192725,GG genotype at ALDH2 rs671 and GG genotype at FOSL1 rs1892901 was lower than that of other genotypes at the corresponding locus,and the differences were s
作者
毛灵
王立晖
MAO Ling;WANG Lihui(Department of Internal Medicine-Oncology,Danyang People's Hospital,Danyang,Jiangsu 212300,China)
出处
《国际检验医学杂志》
CAS
2022年第17期2070-2074,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金青年科学基金项目(31700444)。
