摘要
目的探讨微RNA(miR)-429靶向卵泡抑素样蛋白1(FSTL1)基因对非小细胞肺癌生物学功能的影响。方法收集2020年1月至2021年6月于该院行根治性切除术的60例非小细胞肺癌患者肿瘤组织和癌旁组织,实时荧光定量PCR检测miR-429的表达;按照组织miR-429表达水平依据中位数法进行分组,分为miR-429高表达和miR-429低表达患者,分析其临床特征和预后情况。筛选H1299细胞,分为对照组、miR-429 NC组、miR-429 mimics组,又将pcDNA3.1-con和pcDNA-FSTL1分别转染至高表达miR-429的H1299细胞,分别为miR-429 mimics+pcDNA3.1组和miR-429 mimics+pcDNA-FSTL1组。Transwell检测细胞侵袭,细胞划痕实验检测细胞迁移,双荧光素酶报告实验检测miR-429和FSTL1基因靶向关系。结果非小细胞肺癌组织中miR-429相对表达水平明显低于癌旁组织,非小细胞肺癌细胞系SPCA1、A549、H460、H1299中miR-429相对表达水平明显低于正常肺支气管上皮细胞BESA-2B(P<0.05)。miR-429高表达和低表达患者肿瘤大小、淋巴结转移、TNM分期比较,差异有统计学意义(P<0.05),且miR-429高表达患者生存率明显优于低表达患者。miR-429 mimics组H1299细胞侵袭数量、愈合率明显低于对照组和miR-429 NC组,miR-429 mimics+pcDNA-FSTL1组H1299细胞侵袭数量和愈合率明显高于miR-429 mimics组(P<0.05)。结论miR-429通过靶向FSTL1基因调控非小细胞肺癌的侵袭和迁移。
Objective To investigate the effect of microRNA(miR)-429 targeting follistatin-like protein 1(FSTL1)gene on the prognosis and biological function of non-small cell lung cancer(NSCLC).Methods A total of 60 cases of tumor tissue and para-cancer tissue from patients with NSCLC who underwent radical resection in the hospital from January 2020 to June 2021 were collected.The expression of miR-429 was detected by quantitative real-time PCR.According to the expression of miR-429,the patients were divided into high expression and low expression by median.The clinical characteristics and prognosis of the patients were analyzed.H1299 cells were screened and divided into the control group,the miR-429 NC group and the miR-429 mimics group.PcDNA 3.1-con and pcDNA-FSTL1 were transfected into H1299 cells overexpressing miR-429,which were miR-429 mimics+pcDNA 3.1 group and miR-429 mimics+pcDNA-FSTL1 group,respectively.Transwell assay detected cell invasion,cell scratch assay detected cell migration,and double luciferase reporting assay detected the targeting relationship between miR-429 and FSTL1 gene.Results The relative expression of miR-429 in NSCLC tissues was significantly lower than that in adjacent tissues,and the expression of miR-429 in NSCLC cell lines SPCA1,A549,H460 and H1299 were significantly lower than that in normal lung bronchial epithelial cell BESA-2B(P<0.05).There were significant differences in tumor size,lymph node metastasis and TNM stage between miR-429 high expression and low expression patients(P<0.05),and the survival rate of the patients with miR-429 high expression was significantly better than that of the patients with miR-429 low expression.The invasion number and healing ability of H1299 cells in the miR-429 mimics group was significantly lower than that in the control group and the miR-429 NC group,while the invasion number and healing ability of H1299 cells in the miR-429 mimics+pcDNA FSTL1 group were significantly higher than those in the miR-429 mimics group(P<0.05).Conclusion MiR-429 regulate
作者
郭旭琼
李硕
赵海燕
洪云川
马辉
GUO Xuqiong;LI Shuo;ZHAO Haiyan;HONG Yunchuan;MA Hui(Department of Respiratory,Tianjin Medical University General Hospital,Tianjin 300041,China)
出处
《重庆医学》
CAS
2022年第17期2881-2886,共6页
Chongqing medicine
基金
天津市高等学校科技发展基金计划项目(20130121)。
