摘要
目的研究磷酸二酯酶5(PDE5)基因多态性与阴茎勃起功能障碍(ED)的相关性及其作用机制。方法选取2015年1月~2020年12月我院ED病人107例和健康体检男性102例分别为观察组和对照组,采集两组基本资料,检测PDE5基因多态性以及血清一氧化氮(NO)和环磷酸鸟苷(cGMP)水平并进行比较,分析各指标与勃起功能国际指数5(IIEF-5)评分的关系。结果ED型rs3806808位点T等位基因和TT基因型占比均低于对照组,差异有统计学意义(P<0.05);不同PDE5 rs3806808基因型病人ED严重程度差异显著(P<0.05),TG型和GG型重度病人占比低于TT型,轻、中度病人占比高于TT型,两者相比较差异有统计学意义(P<0.05);不同PDE5 rs3806808基因型病人血清NO水平比较无明显差异(P>0.05),TG型和GG型血清cGMP水平低于TT组,IIEF-5评分高于TT组,且GG型血清cGMP水平低于TG型,IIEF-5评分高于TG型,差异有统计学意义(P<0.05);多元线性回归分析结果显示,血清NO和PDE5 rs3806808基因型为ED病人IIEF-5评分重要影响因素(P<0.05)。结论PDE5 rs3806808基因多态性与ED发病和进展存在密切联系,其作用机制可能主要为影响cGMP表达,与NO表达无明显关系。
Objective To study the correlation between phosphodiesterase 5(PDE5)gene polymorphism and penile erectile dysfunction(ED)and its role mechanism.Methods 107 patients with ED and 102 healthy males with physical examination in the hospital between January 2015 and December 2020 were selected as observation group and control group respectively.The basic data were collected in the two groups,and the PDE5 gene polymorphism and levels of serum nitric oxide(NO)and cyclic guanosine monophosphate(cGMP)were detected and compared,and the relationship between each indicator and International Index of Erectile Function 5(IIEF-5)score was analyzed.Results The proportions of T allele and TT genotype at rs3806808 locus in ED group were lower than those in control group(P<0.05).There was a significant difference in the severity of ED among patients with different genotypes at rs3806808 locus of PDE5(P<0.05).The proportion of severe patients in TG and GG groups was lower than that in TT group,while the proportion of mild-to-moderate patients was higher than that in TT group(P<0.05).There was no significant difference in serum NO level in patients with different genotypes at rs3806808 locus of PDE5(P>0.05),and the level of serum cGMP in TG and GG groups was lower than that in TT group while the IIEF-5 score was higher than that in TT group,and the level of serum cGMP in GG group was lower than that in TG group while the IIEF-5 score was higher than that in TG group(P<0.05).Multivariate linear regression analysis showed that serum NO and genotypes at rs3806808 locus of PDE5 were important factors affecting IIEF score in patients with ED(P<0.05).Conclusion Gene polymorphism of PDE5 rs3806808 locus is closely related to the pathogenesis and progression of ED,and its role mechanism may mainly affect the expression of cGMP,but has no obvious relationship with the expression of NO.
作者
彭辉
贲亮亮
王健
PENG Hui;BEN Liangliang;WANG Jian(The Second People's Hospital of Nantong,Nantong Jiangsu Province,226002,China)
出处
《临床外科杂志》
2022年第7期677-680,共4页
Journal of Clinical Surgery
基金
江苏省卫生健康委科研项目(Z2019025)。
关键词
勃起功能障碍
磷酸二酯酶5
基因多态性
一氧化氮
环磷酸鸟苷
Erectile dysfunction
Phosphodiesterase 5
Gene polymorphism
nitric oxide
cyclic guanosine monophosphate
