摘要
目的 建立高效液相色谱-电喷雾检测器方法(HPLC-CAD)同时测定芪蛭通络胶囊中人参皂苷Rb_(1)和黄芪甲苷的含量。方法 采用色谱柱为Waters XSclectHss T3-C_(18)(4.6 mm×250 mm,5μm),水(A)-乙腈(B)为流动相,梯度洗脱(0 min,15%B;0~10 min,15%~25%B;10~15 min,25%B~31%B;20~50 min,31%B),流速1 mL·min^(-1),柱温25℃,进样量为20μL。电喷雾检测器(CAD)雾化器温度为35℃,功能参数(power function,PF)为1.0。结果 芪蛭通络胶囊中人参皂苷Rb_(1)和黄芪甲苷均具有良好的线性关系(r>0.999 0),定量限(LOQ)分别为0.013和0.015μg,精密度、重复性、24 h稳定性实验的相对标准偏差(RSD)值均小于3.0%,平均加样回收率分别为91.74%和100.09%。结论 本研究建立的HPLC-CAD法可同时对芪蛭通络胶囊中人参皂苷Rb_(1)和黄芪甲苷进行含量测定,为芪蛭通络胶囊的检测分析和质量控制提供了新的方法。
OBJECTIVE To develop a HPLC-CAD method for simultaneous determination of ginsenoside Rb_(1) and astragaloside Ⅳ in Qizhitongluo Capsules.METHODS HPLC analysis was performed on a WatersXSclectHss T3-C_(18) column(4.6 mm×150 mm,5 μm),with mobile phase consisting of water solution(A)-acetonitrile(B) at a flow rate of 1.0 mL· min^(-1 )(0 min,15% B;0-10 min,15%-25%B;10-15 min,25%-31%B;20-50 min,31%B),and column temperature was 25 ℃.The injection volume was 20 μL.The nebulizer chamber temperature for the CAD system was maintained at 35 ℃ and the power function(PF) was 1.0.RESULTS The HPLC-CAD approach developed showed good linearity for ginsenoside Rb_(1) and astragaloside Ⅳ,and the correlation coefficients for calibration curves were both more than 0.9990.The limits of quantitation(LOQ) were found to be 0.013 and 0.015 μg,respectively.The RSDs of precision,repeatability,stability in 24 h were all less than 3.0%.The average recovery rates of ginsenoside Rb_(1) and astragaloside Ⅳ were 91.74% and 100.09%,respectively.CONCLUSION The established HPLC-CAD method can simultaneously determine ginsenoside Rb_(1) and astragaloside Ⅳ in Qizhitongluo capsules,which provides a new approach for the analysis and quality control of Qizhitongluo capsules.
作者
李婷
程显隆
游蓉丽
杨晓宁
李向日
魏锋
马双成
LI Ting;CHENG Xian-long;YOU Rong-li;YANG Xiao-ning;LI Xiang-ri;WEI Feng;MA Shuang-cheng(Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,Center of TCM Processing Research,Beijing University of Chinese Medicine,Beijing 102488,China;National Institutes for Food and Drug Control,Beijing 102629,China;Beijing Zhendong Guangming Pharmaceutical Research Institute Co.,Ltd.,Beijing 100085,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2022年第14期1193-1197,共5页
Chinese Pharmaceutical Journal
