摘要
目的通过电针干预观察对WKY抑郁大鼠行为学及前额叶皮质谷氨酸受体(GLUR1)、N-甲基-D-天冬氨酸(NMDA)受体2B亚基(NR2B)蛋白表达的影响,探讨电针抗抑郁的可能作用机制。方法15只Wistar大鼠分为正常组,另外45只WKY抑郁大鼠随机分为电针组、假针组、模型组,每组15只;电针组取百会和印堂穴连续针刺干预21 d,假针组取穴同电针组,予针刺皮层安慰治疗,正常组与模型组常规饲养,不做干预。记录干预前后各组大鼠体质量、糖水消耗量,并观察旷场实验行为学改变。采用Western blot法检测干预后各组大鼠前额叶皮质GLUR1、NR2B蛋白表达水平。结果电针组、正常组大鼠体质量、旷场试验水平穿越格数与垂直运动次数明显高于假针组和模型组(P<0.01);糖水消耗百分比中电针组、正常组明显高于模型组和假针组(P<0.01);电针组前额叶皮质GLUR1、NR2B蛋白高于模型组(P<0.05,P<0.01)。结论电针可以明显缓解WKY大鼠的抑郁样行为,其作用机制可能与前额叶皮质GLUR1、NR2B蛋白表达增加有关。
Objective To discuss the plausible mechanism of electroacupuncture(EA)in treating depression by observing the effects of EA on the behaviors of Wistar-Kyoto(WKY)depression model rats and the expression of glutamate receptor(GLUR)1 and N-methyl-D-aspartate(NMDA)receptor 2B type(NR2B)proteins in the prefrontal cortex.Method Fifteen Wistar rats were taken as a normal group,and 45 WKY depression model rats were randomized into an EA group,a sham acupuncture group,and a model group,with 15 rats in each group.The EA group received 21 consecutive days of acupuncture treatment selecting Baihui(GV20)and Yintang(GV29).The sham acupuncture group used the same points,but the needles only reached the subcutaneous layer.The normal and model groups received no intervention but only routine feeding.Before and after the intervention,body mass,sugar water consumption,and behaviors in the open field test(OFT)were recorded.The expression levels of GLUR1 and NR2B proteins in the prefrontal cortex of rats were detected using the Western blot method.Result The EA and normal groups surpassed the sham acupuncture and model groups in comparing the body mass and the horizontal crossing and vertical movement times in the OFT(P<0.01).The sugar water consumption percentage was notably higher in the EA and normal groups than in the model and sham acupuncture groups(P<0.01).The expression levels of GLUR1 and NR2B proteins in the prefrontal cortex were higher in the EA group than in the sham acupuncture and model groups(P<0.05,P<0.01).Conclusion EA can significantly ease depressive behaviors in WKY rats,and the mechanism may be associated with increasing the expression of GLUR1 and NR2B proteins in the prefrontal cortex.
作者
李晓慧
秦丽娜
邢龙飞
黄树航
冯荣荣
LI Xiaohui;QIN Lina;XING Longfei;HUANG Shuhang;FENG Rongrong(Beijing University of Chinese Medicine,Beijing 100020,China;Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)
出处
《上海针灸杂志》
2022年第8期818-823,共6页
Shanghai Journal of Acupuncture and Moxibustion
基金
北京中医药大学第三附属医院科研孵化项目(2019kyfh-05)
北京中医药大学新教师启动基金项目(2020-JYB-XJSJJ-066)。
关键词
针刺疗法
电针
抑郁症
行为学
突出可塑性
大鼠
Acupuncture therapy
Electroacupuncture
Depression
Ethology
Synaptic plasticity
Rats
