摘要
目的:通过观察电针“印堂”和“百会”对抑郁模型大鼠大的行为学及海马突触相关蛋白mTOR、GSK-3β(糖原合成酶激酶-3β)的影响,探讨针刺治疗抑郁症的作用机制。方法:正常组15只Wistar大鼠,45只WKY(Wistar-Kyoto)大鼠随机分为电针组、假针组、模型组,治疗前后对所有大鼠进行糖水偏好试验(Sugar Preference Test, SPT)、旷场试验(Open Field Test, OFT)和体重测量。电针组取大鼠“百会穴”“印堂穴”电针治疗,假针组进行假针刺干预,模型组和正常组不进行任何干预。电针组、假针组每天干预1次,干预21天。治疗结束后,用Western Blot法检测大鼠海马mTOR、GSK-3β的表达。结果:和Wistar大鼠相比,干预前WKY大鼠的体重、OFT和SPT评分明显降低(P<0.01)。干预21次后,与模型组及假针组相比,电针组大鼠的体重、OFT和SPT评分明显升高(P<0.01);电针组海马mTOR的表达量要比模型组和假针组更高(P<0.05),且正常组的明显高于模型组(P<0.01);电针组海马GSK-3β的表达量低于模型组和假针组(P<0.05),正常组明显低于模型组(P<0.01)。结论:电针可有效缓解WKY大鼠的抑郁症状,其作用机制可能是通过上调WKY大鼠海马内mTOR蛋白的表达水平,下调GSK-3β蛋白的表达水平,进而改善其突触可塑性,从而改善大鼠的抑郁症状。
Objective:Explore the mechanism of acupuncture on treating the depression by observing the effects of electroacupuncture(EA)at“Baihui”(GV20)and“Yintang”(GV29)on depressive behavior and hippocampal synapse-related protein mTOR and GSK-3β(Glycogen synthase kinase-3β)of WKY rats(Wistar-Kyoto rats,an animal model of depression)Methods:15 Wistar rats served as normal group.45 WKY rats were randomly divided into EA group,Sham Acupuncture(SA)group and model group.Sucrose Water Preference Test(SPT),Open Field Test(OFT)and body mass were measured in all rats before and after the treatment.EA group were treated with GV20 and GV29,SA group were treated with sham acupuncture.Fed model group and normal group were regularly raised without any intervention.After the treatment,we detect the Protein expression of mTOR and GSK3-βin rat hippocampus synapsis by Western blot.Results:Before the treatment,WKY rats have higher weight and perform better in SPT and OFT,compared with Wistar rats(P<0.01).After the treatment,EA group have higher weight,SPT rate and OFT rate compare among SA group and model group.(P<0.01).The expression level of mTOR in hippocampus in EA group was higher,compared among SA group and model group(P<0.05).The expression level of mTOR in hippocampus in normal group was higher,compared with model group(P<0.01).The expression level of GSK3-βin hippocampus in EA group was lower,compared among SA group and model group(P<0.05).The expression level of GSK3-βin hippocampus in normal group was lower,compared with model group(P<0.01)Conclusion:The depression symptoms of WKY rats were relieved by electroacupuncture treatment.Its mechanism could be related to the up-regulating expression of mTOR in hippocampus and the down-regulating expression of GSK3-β,these changes of protein expression can improve the synaptic plasticity in hippocampus and therefore alleviate depression.
作者
黄树航
邢龙飞
杨帅
李晓慧
冯荣荣
王子未
秦丽娜
HUANG Shuhang;XING Longfei;YANG Shuai(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Beijing Daxing District Hospital of Integrated Chinese and Western Medicine,Beijing 100163,China)
出处
《四川中医》
2023年第11期75-80,共6页
Journal of Sichuan of Traditional Chinese Medicine
基金
北京中医药大学第三附属医院科研孵化项目(编号:2019kyfh-05)
北京中医药大学校级课题(编号:2020-JYB-XJSJJ-066)。
关键词
电针
抑郁症
突触可塑性
electroacupuncture
depression
synaptic plasticity
