摘要
目的探讨微小核糖核酸(micro RNA,miR)-448和赖氨酸特异性脱甲基酶2B[lysine(K)-specific demethylase 2B,KDM2B]在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)组织中的表达及临床意义。方法选择2017年7月~2018年3月在达州市中心医院收治的97例DLBCL患者作为观察对象,选取同期50例淋巴结反应性增生(reactive lymph node hyperplasia,RHL)组织患者作为对照组,采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)法检测DLBCL组织与RHL组织中miR-448和KDM2B mRNA表达水平,免疫组织化学染色法检测KDM2B蛋白表达水平,分析miR-448和KDM2B表达水平与DLBCL患者临床病理特征的关系,Pearson分析miR-448和KDM2B表达水平的相关性,Kaplan-Meier生存分析miR-448和KDM2B表达与DLBCL患者生存率的关系以及COX回归模型分析影响DLBCL患者预后的影响因素。结果DLBCL组织中miR-448表达水平为0.65±0.08,显著低于对照组的1.04±0.19,差异有统计学意义(t=17.473,P<0.001);DLBCL组织中KDM2B mRNA表达水平为2.89±0.54,显著高于对照组的0.98±0.21,差异有统计学意义(t=24.058,P<0.001);免疫组织化学染色结果显示,与对照组比较,DLBCL组织中KDM2B蛋白阳性表达率(72.16%vs 22.00%)显著升高,差异有统计学意义(χ^(2)=33.561,P<0.001);miR-448和KDM2B表达与Ann Arbor分期、IPI评分、Hans分型有关(χ^(2)=4.227~12.960,均P<0.05);与年龄、性别、LDH水平、ECOG评分和有无B症状无关(χ^(2)=0.072~3.726,均P>0.05);相关性分析显示,miR-448和KDM2B表达水平呈负相关(r=-0.602,P<0.001);Kaplan-Meier生存分析显示,miR-448高表达组DLBCL患者三年累积生存率显著高于miR-448低表达组患者(55.53%vs 31.38%),差异有统计学意义(χ^(2)=7.434,P=0.006)。KDM2B阳性表达组DLBCL患者三年累积生存率显著低于阴性表达组患者(31.73%vs 56.67%),差异有统计学意义(χ^(2)=8.877,P=0.006);COX多因素分析结果显示,Ann Arbor分期(95%CI=1.567~4.012,P=0.005),miR-448(95%CI=2.213~4.478,P=
Objective To investigate the expression and clinical significance of microRNA(miR)-448 and lysine(K)-specific demethylase 2B(KDM2B)in diffuse large B-cell lymphoma(DLBCL)tissues.Methods A total of 97 DLBCL patients who were admitted to Dazhou Central Hospital from July 2017 to March 2018 were selected as the observation object,and 50 patients with reactive hyperplasia of lymph node(RHL)during the same period were selected as the control group.The expression levels of miR-448 and KDM2B mRNA in DLBCL tissues and RHL were detected by quantitative real-time PCR(qRT-PCR),the expression level of KDM2B protein was detected by immunohistochemical staining method,the relationship between the expression levels of miR-448,KDM2B and clinicopathological characteristics in DLBCL patients was analyzed,the relationship between the expression of miR-448,the correlation between the expression levels of miR-448 and KDM2B was detected by Pearson analysis.KDM2B and the survival rate of DLBCL patients was detected by Kaplan-Meier survival analysis,and COX regression model was used to analyse the influencing factors of prognosis in patients with DLBCL.Results The expression level of miR-448 in DLBCL tissue was 0.65±0.08,which was significantly lower than 1.04±0.19 in the control group,and the difference was statistically significant(t=17.473,P<0.001).The expression level of KDM2B mRNA in DLBCL tissue was 2.89±0.54,which was significantly higher than 0.98±0.21 of the control group,the difference was statistically significant(t=24.058,P<0.001).The results of immunohistochemical staining showed that compared with the control group,the positive expression rate of KDM2B protein in DLBCL(72.16%vs 22.00%)tissue was significantly increased,the difference was statistically significant(χ^(2)=33.561,P<0.001).The expression of miR-448 and KDM2B was related to Ann Arbor staging,IPI score and Hans classification(χ^(2)=4.227~12.960,all P<0.05).But not related to age,gender,LDH level,ECOG score,and the presence or absence of B symptom(χ^(2)=0.0
作者
陈思言
张伶莉
杨丽华
CHEN Si-yan;ZHANG Ling-li;YANG Li-hua(Department of Hematology,Dazhou Central Hospital,Sichuan Dazhou 635000,China)
出处
《现代检验医学杂志》
CAS
2022年第4期128-133,共6页
Journal of Modern Laboratory Medicine
