摘要
背景人体呼吸道和胃肠消化道具有相似的黏膜结构,黏膜屏障构成机体抵御外邪的第一道防线,当呼吸道感染后刺激黏膜产生局部免疫应答,通过迁移和归巢影响到肠道,导致黏膜局部抗感染能力减弱,诱发黏膜屏障损伤,加重慢性阻塞性肺疾病(COPD)肺部病变。目的基于“肺与大肠相表里”中医药经典脏腑理论,观察补肺健脾方对COPD稳定期模型大鼠肺组织炎性因子表达水平,肺、结肠组织肺肠相关活性肽表达水平的影响。方法2019年9月至2020年12月选取SPF级SD大鼠50只,采用乱数表法将50只大鼠随机分为对照组、模型组、补肺健脾组、氨茶碱组和益生菌组,每组10只。1~8周采用香烟烟雾暴露联合鼻腔滴注脂多糖(LPS)制作COPD模型(对照组除外)。各组自第9周起灌胃给药,12周结束后取材。在光镜下观察肺、结肠组织病理变化,采用免疫组化法检测肺组织肿瘤坏死因子α(TNF-α)、白介素10(IL-10)表达水平,酶联免疫吸附测定(ELISA)法检测支气管肺泡灌洗液(BALF)分泌型免疫球蛋白A(SIgA)及肺、结肠组织P物质(SP)、血管活性肠肽(VIP)表达水平。结果对照组大鼠肺、结肠组织结构基本完整;模型组大鼠气管周围存在大量炎性细胞,支气管管壁增厚,气管变窄,补肺健脾组改善肺组织病理效果最为显著;模型组大鼠结肠组织出现损伤,表现为大量上皮细胞脱落,隐窝形态大小不一,补肺健脾组改善结肠组织病理效果良好。五组大鼠肺组织TNF-α、IL-10、SP、VIP表达水平比较,差异均有统计学意义(F_(TNF-α)=70.640、F_(IL-10)=8.444、F_(SP)=108.700、F_(VIP)=4.665,P<0.05);五组大鼠BALF中SIgA表达水平比较,差异有统计学意义(F=26.370,P<0.05);五组大鼠结肠组织SP、VIP表达水平比较,差异均有统计学意义(F_(SP)=136.600,F_(VIP)=13.980,P<0.05)。与对照组相比,模型组大鼠肺组织TNF-α、SP表达水平与结肠组织SP、VIP表达水平均升高(P<0.0
Background Themucosal structure of the respiratory tract is similar to that of the gastrointestinal tract in humans,which is the body's first line of defense against the invasion of pathogens through the function of mucosal barrier.When the respiratory tract infection stimulates the airway mucosa to produce a local immune response,it affects the intestinal tract through migration and homing,resulting in the weakening of the local mucosal anti-infection ability,inducing mucosal barrier damage,and further aggravating chronic obstructive pulmonary disease(COPD).Objective To explore the effects of Bufei Jianpi Formula on the expression levels of inflammatory factors in lung tissue,and the content of lung-gut related peptides in lung and colon tissues in a rat model of stable COPD based on a typical viscera theory of Traditional Chinese Medicine,namely“the lungs and large intestines are interior-exteriorly related”.Methods This experiment was carried out from September 2019 to December 2020.Fifty SPF SD rats were selected,and equally and randomly divided into control,model,Bufei Jianpi,aminophylline and probiotics groups.Except for the control group,the rats in other groups received cigarette smoke exposure combined with intranasal instillation of lipopolysaccharide(LPS)in the first eight weeks of intervention for establishing a COPD model.From the ninth week of intervention,rats in the control and model groups received intragastric administration of 0.9%sodium chloride solution,and those in Bufei Jianpi,aminophylline and probiotics groups received intragastric administration of Bufei Jianpi Formula,aminophylline and probiotics,respectively.The rats were sacrificed after 12 weeks of intervention.The pathological changes of lung and colon tissues were observed under an optical microscope,the expression levels of tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)in lung tissue were detected by immunohistochemistry.The contents of secretory immunoglobulin A(SIgA)in bronchoalveolar lavage fluid(BALF),substance
作者
张丛丛
李亚
李素云
毛静
ZHANG Congcong;LI Ya;LI Suyun;MAO Jing(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Chinese Medicine Pharmacology(Respiratory)Laboratory,the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province&Education Ministry of P.R.China,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中国全科医学》
CAS
北大核心
2022年第30期3761-3767,共7页
Chinese General Practice
基金
国家自然科学基金资助项目(81904116)
中医药传承与创新“百千万”人才工程(岐黄工程)项目岐黄学者([2018]284号)。
关键词
肺疾病
慢性阻塞性
免疫
黏膜
血管活性肠肽
补肺健脾方
大鼠
Pulmonary disease,chronic obstructive
Immunity,mucosal
Vasoactive intestinal peptide
Bufei Jianpi Formula
Rats
