摘要
MYB转录因子家族广泛参与了植物对干旱、盐渍、冷害等非生物胁迫的应答。为了深入研究秋葵[Abelmoschus esculentus(L.) Moench]中的MYB类转录因子,该研究以‘北海道1号’秋葵为研究对象,采用PCR方法克隆AeMYB1R1基因,并借助生物信息学进行特征分析;采用qRT-PCR荧光定量方法分析其表达模式及其在非生物胁迫下的表达特性。结果表明:(1)成功克隆获得1个秋葵AeMYB1R1基因;该基因包含1个1 056 bp的开放阅读框,编码352个氨基酸;序列对比和系统进化树结果显示,AeMYB1R1在植物进化过程中具有较高的保守性;AeMYB1R1蛋白分子量为37 891.57 Da,等电点为8.75,含有较多的谷氨酸和较少的色氨酸,以及较多潜在的磷酸化位点和糖基化位点。(2)结构分析显示,AeMYB1R1蛋白主要由α螺旋和无规则卷曲构成,无信号肽和跨膜结构,为疏水性蛋白;同时,氨基酸序列在第104至第156位含有一个保守结构域,表明其属于SHAQKYF类MYB家族转录因子。(3)qRT-PCR结果显示,AeMYB1R1基因在秋葵叶中的表达量最高,其次是根和茎,具有组织表达特性;与高温和低温胁迫相比,在盐胁迫和干旱胁迫中AeMYB1R1表达量更高,说明AeMYB1R1可能是秋葵抗盐和抗旱的关键转录因子。研究结果为AeMYB1R1基因在秋葵生长发育和抗逆机制中的功能研究奠定了理论依据。
MYB transcription factor family is widely involved in plant response to abiotic stresses, including drought, salinity, and chilling. To further study MYBs in okra [Abelmoschus esculentus(L.) Moench], in this study, we cloned a gene of AeMYB1R1 from ‘Hokkaido No.1’ of okra by PCR and characterized using bioinformatic analysis. Quantitative real-time PCR(qRT-PCR) was used to analyze AeMYB1R1 expression pattern and the expression characterization under abiotic stresses. The results showed that:(1) AeMYB1R1 was successfully cloned from okra, which contained an open reading frame of 1 056 bp, encoding 352 amino acids. Sequence alignment and phylogenetic tree analysis showed that, AeMYB1R1 was evolutionarily highly conserved in plants. AeMYB1 R1 had a molecular weight of 37 891.57 Da and an isoelectric point of 8.75, which contained more glutamic acid and less tryptophan, as well as more potential phosphorylation sites and glycosylation sites.(2) Structural analysis showed that AeMYB1R1 was mainly dominated by α-helix and random coil, without signal peptide and transmembrane structure, which was a hydrophobic protein. Meanwhile, its amino acid sequence contained a conserved domain from 104 to 156, indicating that it belonged to the SHAQKYF class of MYBs.(3) qRT-PCR results showed that AeMYB1R1 had the highest expression level in okra leaf, followed by root and stem, which reflected its tissue-specific expression pattern. Meanwhile, compared with heat and cold stress, AeMYB1R1 was highly expressed in response to salt stress and drought stress, which suggested that AeMYB1R1 may be the key transcription factor for salt and drought resistance of okra. In summary, this study lays a theoretical basis for the functional study of the AeMYB1R1 gene in okra growth and development and in the mechanism of stress resistance.
作者
朱志鹏
刘慧玲
吴可鑫
虞健翔
王博文
孙淼
ZHU Zhipeng;LIU Huiling;WU Kexin;YU Jianxiang;WANG Bowen;SUN Miao(Jiangsu Key Laboratory for Bioresources of Saline Soils,College of Marine and Biological Engineering,Yancheng Teachers University,Yancheng,Jiangsu 224002,China;State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第6期909-919,共11页
Acta Botanica Boreali-Occidentalia Sinica
基金
江苏省盐土生物资源研究重点实验室开放项目(JKLBS2016002)
江苏省高等学校自然科学研究面上项目(21KJB210007)。
关键词
秋葵
AeMYB1R1
非生物胁迫
Abelmoschus esculentus(L.)Moench
AeMYB1R1
abiotic stress
