摘要
GNOM是一种ADP核糖基化因子(ARF)的鸟嘌呤核苷酸交换因子(GEF),为探索GNOM在香鳞毛蕨(Dryopteris fragrans)中的抗逆功能,该研究克隆了DfGNOM并进行生物信息学分析,采用实时荧光定量PCR(qRT-PCR)方法分析了DfGNOM基因在不同植物激素及逆境胁迫处理下的表达模式,为进一步探索该基因的功能以及香鳞毛蕨的抗逆机制奠定基础。结果表明:(1)成功获得DfGNOM全长4338 bp,蛋白质多序列比对以及进化树分析表明,DfGNOM与江南卷柏(Selaginella moellendorffii)SmGNOM亲缘较近,motif分析表明该蛋白含有sec7保守结构域。(2)qRT-PCR分析显示,DfGNOM在香鳞毛蕨的根、叶柄和叶中均有表达,但在叶中表达量最高;DfGNOM的相对表达量在生长素(IAA)处理后总体上调,在脱落酸(ABA)处理后总体下调;在NaCl处理下呈“降-升-降”的变化趋势,高温和低温处理下呈“升-降-升”变化趋势;在茉莉酸甲酯(MeJA)、乙烯利(ETH)以及PEG处理下,DfGNOM的相对表达也表现出不同的模式。研究认为,DfGNOM在香鳞毛蕨非生物胁迫响应过程中发挥调控作用。
GNOM is a kind of guanine nucleotide exchange factor(GEF)of ADP ribosylation factor(ARF).In order to explore the function of GNOM in stress resistance of Dryopteris fragrans,we cloned DfGNOM and analyzed by bioinformatics.The expression pattern of DfGNOM gene under different plant hormones and stress was analyzed by real-time fluorescent quantitative PCR(qRT-PCR).The results provide a basis for further exploring the function of the gene and the stress resistance mechanism of D.fragrans.The results showed that:(1)DfGNOM was obtained with a total length of 4338 bp.Multiple protein sequence alignment and phylogenetic tree analysis showed that DfGNOM was closely related to Selaginella moellendorffii SmGNOM,and motif analysis showed that the protein contained the sec7 conserved domain.(2)qRT-PCR analysis showed that:DfGNOM was expressed in the roots,petioles and leaves of D.fragrans,but the highest expression was in the leaves;the relative expression of DfGNOM was up-regulated after IAA treatment;abscisic acid(ABA)treatment decreased the expression level;under NaCl treatment,the trend was“down-up-down”;under high temperature and low temperature treatment,the trend was“up-down-up”.The relative expression patterns of DfGNOM were also different under the treatments of MeJA,ETH and PEG.The results showed that DfGNOM played a regulatory role in the abiotic stress response of D.fragrans.
作者
邱晓杰
汤珣
官亚琳
张冬瑞
苏颖
常缨
QIU Xiaojie;TANG Xun;GUAN Yalin;ZHANG Dongrui;SU Ying;CHANG Ying(College of Life Sciences, Northeast Agricultural University, Harbin 150030, China;Nanshan Affiliated School of Beijing Normal University, Shenzhen, Guangdong 518000, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2021年第8期1279-1286,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31870313)。
