摘要
LBD是一类具有LOB（lateral organ boundaries）结构域的基因家族,在植物发育过程中起到非常重要的作用。采用生物信息学方法,根据拟南芥LBD基因序列鉴定了普通烟草基因组中的LBD基因,并对家族成员进行了序列特征、系统发育和表达谱分析。结果表明：普通烟草基因组中共有98个LBD基因成员,其基因结构相对简单,一般含有1~3个外显子。LBD基因家族可分成I和II两大类,两类均含有CX_2CX_6CX_3C保守结构域,但II类不含有LX_6LX_3LX_6L形成的＂卷曲螺旋＂二级结构,根据与拟南芥LBD蛋白构建的系统发育树则可细分成5个亚家族（Ia、Ib、Ic、Id和II）。将LBD基因与表达序列标签（EST）比对,发现36个基因有EST证据;EST、芯片数据和转录组数据分析表明：LBD基因具有不同的组织表达模式,部分基因表现出组织特异性。这些研究结果为普通烟草LBD基因家族功能的深入研究奠定了基础。
LBD gene with LOB（ lateral organ boundaries） domain plays very important role in plant development. Base on bioinformatic method,the LBD genes of Nicotiana tabacum were screened with the sequences of Arabidopsis. The sequence structure,phylogenetic relationship and expression pattern of the identified gene sequences were analyzed. The result show that a total of 98 LBD genes were identified from tobacco with simple genetic structures contained 1-3 exons generally. LBD gene family was divided into two types（ I and II）. All LBD members had conserved domain CX_2CX_6CX_3C,however,the type II genes didnot had secondary structure of coil made of LX_6LX_3LX_6 L. Furthermore,LBD genes were divided into 5 subfamilies（ Ia,Ib,Ic,Id and II） by their phylogenetic relationship with Arabidopsis. A total of 36 members had EST evidence based on the comparison about LBD genes and EST sequences. The analysis of EST,array data and transcriptome showed that the LBD genes had different expression patterns and tissue specificity. These results provided abundant information for further functional analysis of the LBD genes family.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2016年第2期316-325,共10页
Journal of Plant Genetic Resources
基金
国家高技术研究发展计划("863"计划)项目(2012AA021801)
关键词
普通烟草
LBD基因家族
系统进化
基因表达
Nicotiana tabacum
LBD gene family
phylogenetic evolution
gene expression
