摘要
利用生物信息学方法,对萝卜全基因组SUS基因家族成员进行鉴定与表达分析,结果表明:1)从萝卜基因组中鉴定出7个SUS基因家族成员,且每个家族成员均包含3个保守基序,外显子数量为11~14个。2)根据进化树聚类结果,7个SUS基因家族成员可分为3大类,即SUSⅠ、SUSⅡ和SUSⅢ,它们分别包含2个、2个和3个SUS基因家族成员。3)这7个SUS基因家族成员分布在4条染色体上,7号染色体上分布有3个SUS基因家族成员,9号染色体上分布有2个SUS基因家族成员,3号和4号染色体分别有1个SUS基因家族成员。4)萝卜SUS基因家族成员启动子包括参与激素响应、参与环境响应和抗性、参与厌氧诱导及分生组织表达的顺式作用元件。5)RsSUS1基因的2个拷贝(即Rsa10035816和Rsa10021071)在萝卜不同组织和不同发育时期的表达量均较高。以上研究为萝卜SUS基因家族成员的生物学功能分析提供了基础。
The paper uses bioinformatics methods to identify and analyze the expression of SUS gene family members in radish genome.The results show that:1)Seven SUS gene family members are identified from radish genome,and each family member contains three conserved motifs with 11~14 exons.2)According to the phylogenetic tree clustering results,the seven SUS gene family members can be divided into three categories,namely SUSⅠ,SUSⅡ,SUSⅢ,which contains two,two and three SUS family members respectively.3)The seven SUS gene family members are distributed on four chromosomes,three SUS gene family members are distributed on chromosome 7,two SUS gene family members are distributed on chromosome 9,and one SUS gene family member is distributed on chromosome 3 and 4 respectively.4)The promoter of SUS gene family members in radish includes cis acting elements involved in hormone response,environmental response and resistance,anaerobic induction and meristem expression.5)Two copies of RsSUS1(Rsa10035816 and Rsa10021071)are highly expressed in different tissues and developmental stages of radish.Thus,these results will be helpful for the functional characterization of the SUS genes family.
作者
刘同金
胡海琳
张爱慧
刘良峰
王长义
LIU Tong-jin;HU Hai-lin;ZHANG Ai-hui;LIU Liang-feng;WANG Chang-yi(Jinling Institute of Technology, Nanjing 210038, China;Agricultural and Rural Bureau of Jiangning District, Nanjing 211100, China)
出处
《金陵科技学院学报》
2021年第2期80-87,共8页
Journal of Jinling Institute of Technology
基金
国家自然科学基金(31801858)
金陵科技学院“科教融合”项目(2020KJRH39)
金陵科技学院高层次人才科研启动基金(jit-b-202009)。
