摘要
目的 观察白藜芦醇对卵巢早衰小鼠的干预作用,并阐述其可能机制。方法 40只两月龄雌性小鼠随机分为4组,分别为对照组(Control)、模型组(Model group)、20 mg/kg RES组(20 mg/kg RES group)和40 mg/kg RES组(40 mg/kg RES group),每组10只。测量小鼠体重、卵巢重量和卵巢/小鼠相对重量比;采用HE染色观察小鼠卵巢组织中卵泡数量变化;RT-qPCR法检测卵巢组织中CyclinD1、Wnt1和β-catenin mRNA表达;免疫组化染色观察卵巢组织中β-caterin蛋白表达;Western blot法检测卵巢组织中Mvh、Oct4、SOD2、Nrf2、Bax和Bcl-2蛋白表达。结果 20 mg/kg RES组和40 mg/kg RES组小鼠体重、卵巢重量和卵巢/小鼠重量比均高于模型组;与模型组比较,20 mg/kg RES组和40 mg/kg RES组小鼠原始卵泡数量增加、闭锁卵泡数量下降(P<0.05),而卵巢组织中CyclinD1、Wnt1和β-catenin mRNA表达增加(P<0.05);与模型组比较,20 mg/kg RES组和40 mg/kg RES组小鼠卵巢组织中β-caterin阳性蛋白表达升高,且呈浓度依赖性;与模型组比较,20 mg/kg RES组和40 mg/kg RES组小鼠卵巢组织中Mvh、Oct4、SOD2、Nrf2和Bcl-2蛋白表达升高,Bax表达下降(P<0.01)。结论 白藜芦醇对卵巢早衰小鼠具有保护作用,其机制可能与抑制卵巢细胞凋亡,激活Wnt/β-catenin通路促进卵巢功能恢复有关。
Objective To observe the protective effect of resveratrol in mice with premature ovarian failure and to explore its possible mechanism. Methods Forty two-month-old female mice were randomly divided into four groups: control group(Control), model group, 20 mg/kg RES group, and 40 mg/kg RES group with 10 mice in each group. Changes in body weight and the ovarian weight coefficient were assessed. Follicle number changes in mouse ovarian tissue were observed by HE staining. Relative mRNA expression of cyclin D1, Wnt1 and β-catenin in ovarian tissues was measured by RT-qPCR. β-catenin protein expression in ovarian tissue was detected by immunohistochemical staining. Mvh, Oct4, SOD2, Nrf2, Bax and Bcl-2 protein expression in ovarian tissues was detected by Western blot. Results Body weight, ovary weight, and ovary/mouse weight ratio in 20 and 40 mg/kg RES groups were higher than those in the model group. Compared with the model group, the number of primordial follicles was increased and the number of atresia follicles was decreased in 20 and 40 mg/kg RES groups(P<0.05), while mRNA expression of cyclin D1, Wnt1 and β-catenin was increased in ovarian tissues(P<0.05). Compared with the model group, β-catenin protein expression was increased in ovarian tissues in 20 and 40 mg/kg RES groups in a concentration-dependent manner. Compared with the model group, Mvh, Oct4, SOD2, Nrf2 and Bcl-2 protein expression was increased and Bax expression was decreased in ovarian tissues of 20 and 40 mg/kg RES groups(P<0.01). Conclusions The protective effect of resveratrol in mice with premature ovarian failure may be related to inhibition of ovarian cell apoptosis and activation of the Wnt/β-catenin pathway to promote recovery of ovarian functions.
作者
修银玲
孙凯旋
于月新
XIU Yinling;SUN Kaixuan;YU Yuexin(Department of Reproductive Medicine,General Hospital of the Northern Theater,Shenyang 110000,China)
出处
《中国比较医学杂志》
CAS
北大核心
2022年第7期81-86,93,共7页
Chinese Journal of Comparative Medicine
基金
辽宁省科学技术计划项目(2020JH2/10300118)。
关键词
白藜芦醇
卵巢早衰
小鼠
卵巢
卵泡
resveratrol
premature ovarian failure
mice
ovary
follicle
