摘要
目的在活体状态下应用CT灌注扫描技术,评价大鼠急性脑缺血再灌注模型的可靠性。方法利用线栓法建立大鼠急性脑缺血再灌注模型,并通过其CT灌注扫描图像与脑切片TTC染色图像相比较对模型进行评价。结果大脑中动脉栓塞后2 h,CT灌注扫描显示明确的缺血范围。持续栓塞组大鼠脑切片TTC染色均显示明显的脑梗死灶,与CT灌注扫描显示的缺血范围具有一致性;再通组大鼠回撤栓线后,栓塞侧脑血流能够恢复,TTC染色未发现明显梗死灶。结论线栓法制作可复性大鼠急性脑缺血模型稳定可靠,操作简单。如能用CT灌注扫描对梗死模型进行筛选则可进一步降低由模型变异引起的实验误差,从而为脑梗死的治疗研究提供可靠平台。
Objective To evaluate the reliability of a rat model of acute cerebral ischemia and reperfusion by using cerebral perfusion functional CT. Methods A stable and reversible focal ischemia model with unilateral middle cerebral artery occlusion was established and evaluated by CT perfusion imaging and TTC staining. Results Artificial Occlusion of the MCA resulted in ipsilateral cerebral infarcts in all study animals. Hypoperfusion was definitely recorded in all CT perfusion images obtained after MCA occlusion and was significantly correlated with the final lesion size. Blood flow was restored after pulling the thread out of the artery. Conclusions The method of establishing an acute focal cerebral ischemia and reperfusion model by thread insertion in our study is simple and stable, ff we can screen the stroke model with CT perfusion examination, the error caused by variance of model can be reduced. Thereby it provides a platform for researchers to investigate acute cerebral ischemia and recirculation.
出处
《基础医学与临床》
CSCD
北大核心
2006年第6期631-634,共4页
Basic and Clinical Medicine
关键词
脑缺血
再灌注
模型
灌注CT
cerebral ischemia
reperfusion
models
perfusion CT
