摘要
测定水体中的氨氮含量是本科生实验教学和科研中常见的实验项目。传统方法如纳氏比色法、吲哚酚蓝(IPB)比色法、水杨酸比色法中学生会接触到有毒、有味或高腐蚀性的物质,且操作过程繁琐,给教学带来潜在的安全风险。根据最近新报道的一种氨氮测定方法,设计教学实验并绘制了标准曲线。结果显示,此方法经30次重复操作,均制得R大于0.99的标准曲线,且各组数据RSD值均小于3%,加标回收率在100.9%~109.5%之间,具有较高的准确度、精密度和可重复性。另外,该方法安全性高、仪器简单、操作步骤简便、显色稳定,可在本科教学和科研中推广。在研究中还发现了该方法的干扰因素为三价铁离子和氨基酸,因此在测量富含这两种物质的水体时要预先将其去除。
Determining the content of ammonia nitrogen in water is a common experimental item in experimental teaching and scientific research for undergraduates.Traditional methods such as Nessler’s colorimetric method,indoxyl blue(IPB)colorimetric method,and salicylic acid colorimetric method mayexpose students to toxic,odorous or highly corrosive substances,and the process is cumbersome.Teaching brings potential unsafe factors.Recently,a new method for the determination of ammonia nitrogen was reported.We arranged for students of different grades to operate anddraw a standard curve.The results show that after 30 repeated operations,this method produced a standard curve with R~2 greater than 0.99,and the RSD values of each group of data were all less than 3%,the recovery rate of standard addition was between 100.9%and 109.5%,which has high accuracy,precision and repeatability.In addition,the method has the advantages of safety,non-toxicity,simple instrument,simple operation steps,stable color rendering,etc.It is worthy of popularization in undergraduate teaching and scientific research.This study found that the interference factors of this method are ferric ions and amino acids,so they must be removed in advance asmeasuring water rich in these two substances.
作者
孔令明
陈知雨
徐瑜聪
贾昌锋
KONG Lingming;CHEN Zhiyu;XU Yucong;JIA Changfeng(Marine College,Shandong University,Weihai 264209,Shandong,China)
出处
《实验室研究与探索》
CAS
北大核心
2022年第4期191-194,235,共5页
Research and Exploration In Laboratory
基金
山东省自然科学基金项目(ZR2019PC027)。
关键词
氨氮测定
吲哚酚蓝
干扰因素
安全无毒
本科生实验教学
ammonia nitrogen detection
indophenol blue(IPB)
interference factors
safety and nontoxicity
experiment teaching for undergraduates
