摘要
为研究白背叶楤木(Aralia chinensis Linn.var.nuda Nakai)根、茎、叶部位的挥发油成分组成及抗氧化活性,采用水蒸气蒸馏法提取白背叶楤木3个部位挥发油,运用气相色谱-质谱联用法(GC-MS)对各部位挥发油的化学成分进行分析和鉴定,并以DPPH和ABTS自由基清除能力为指标评价各部位挥发油的抗氧化作用。结果显示白背叶楤木各部位挥发油成分及含量存在较大差异,从三个部位挥发油共鉴出化学成分123种,根鉴出49种,醇类占比最大(24.33%);茎鉴出39种,有机酸类物质占比最大(33.82%);叶鉴出73种,醇类占比最大(44.64%)。抗氧化实验中,根部挥发油表现出较好的抗氧化活性,清除DPPH自由基和ABTS自由基的IC_(50)分别为4.97、2.90mg/mL,茎挥发油和叶挥发油抗氧化活性较弱。
In order to investigate the chemical components and antioxidant activity of essential oil from the roots,stems and leaves of Aralia chinensis Linn.var.nuda Nakai,the essential oil was extracted by steam distillation and the chemical constituents in three parts were analyzed and identified by gas chromatography-mass spectrometry(GC-MS).The antioxidant effect of essential oil was evaluated by scavenging capacities against DPPH and ABTS free radicals.Results demonstrated that differences in the compositions and contents of essential oil in different parts of A.chinensis var.nuda were significant.123 chemical compounds were identified from essential oil in three parts of A.chinensis var.nuda,48 compounds were identified in roots,among which alcohols accounted for the largest proportion(24.33%);39 compounds were identified in stems,which major compounds were organic acids(33.82%),and 73 compounds in leaves,alcohols were main components(44.64%).In antioxidant experiments,the essential oil from the roots showed significant antioxidant activity with IC_(50) of 4.97mg/mL to DPPH and 2.90mg/mL to ABTS,the stems and leaves essential oil showed weaker antioxidant activity.
作者
尹芃程
王志尧
王亭
钱海珊
李艳平
董发武
李宝晶
何红平
YIN Pengcheng;WANG Zhiyao;WANG Ting;QIAN Haishan;LI Yanping;DONG Fawu;LI Baojing;HE Hongping(Yunnan Key Laboratory of Southern Medicinal Utilization,College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500;Henan Natural Product Biotechnology Co.,Ltd.,Henan Academy of Science,Zhengzhou 450002;Yunnan Key Laboratory of Dai and Yi Medicine,College of Ethnic Medicine,Yunnan University of Chinese Medicine,Kunming 650500)
出处
《中国食品添加剂》
CAS
北大核心
2022年第8期170-179,共10页
China Food Additives
基金
国家自然科学基金(81860760
81760745)
云南省重大项目专项(202002AA10000)
云南省应用基础研究计划(2019FB117)
云南省应用基础研究计划中医联合专项[2018FF001(-024)]
河南省科学院基本科研费项目(220613048)
河南省重点研发与推广专项(212102310352)。
关键词
白背叶楤木
挥发油
气相色谱-质谱联用法(GC-MS)
成分分析
抗氧化
Aralia chinensis Linn.var.nuda Nakai
essential oil
gas chromatography-mass spectrometry(GCMS)
component analysis
antioxidant activity
