摘要
通过40%CCl_4橄榄油剂3 mL·kg^(-1)腹腔注射,2次/周,连续8周诱导的肝纤维化大鼠模型,观察太白楤木对CCl_4诱导的肝纤维化模型大鼠肝组织中血管内皮生长因子(VEGF)、转化生长因子-β1(TGF-β1)mRNA表达水平及Bcl-2,Bax蛋白表达水平的影响。取雄性SD大鼠60只,将选取的60只SD大鼠随机分为正常组,模型组,太白楤木高、中、低剂量组(10,7.5,5.0 mL·kg^(-1))和秋水仙碱组。HE及Masson染色观察肝组织病理学改变;RT-PCR检测VEGF,TGF-β1 mRNA表达水平;Western blot检测Bcl-2,Bax蛋白表达水平。实验研究发现:模型组可见明显的肝细胞变性、坏死,大量胶原纤维组织增生,部分可见假小叶形成;太白楤木各剂量组及秋水仙碱组肝细胞变性坏死减轻,胶原纤维组织增生减少,未见明显假小叶形成。与模型组相比,太白楤木各剂量组及秋水仙碱组VEGF mRNA表达均有不同程度减低(P<0.05),太白楤木高、中剂量组TGF-β1mRNA表达减低(P<0.05);太白楤木高、中剂量组VEGF mRNA表达较秋水仙碱组减低(P<0.01);太白楤木高剂量组TGF-β1mRNA表达较秋水仙碱组减低(P<0.05)。与模型组相比,太白楤木各剂量组及秋水仙碱组Bcl-2蛋白表达均减低(P<0.05),Bax蛋白表达则升高(P<0.05)。太白楤木具有良好的抗肝纤维化作用,可能是通过抑制VEGF,TGF-β1等细胞因子的表达,同时上调肝组织中Bax蛋白及下调Bcl-2蛋白的表达,以调节肝组织胶原纤维代谢进而改善肝脏纤维化的程度。
[Abctract]Hepatic fibrosis models were induced by CCl_4 in rats.To explore vascular endothelial growth factor（VEGF）,transforming growth factor-beta1（TGF-β1） mRNA expression and bcl-2,Bax protein expression levels of intervention and explore the mechanism of the Aralia chinesis anti-hepatic fibrosis.Sixty male Sprague-Dawlley（SD） rats were randomly divided into six groups：nomal group,model group,high-dose（10 mL·kg^（-1））,medium-dose（7.5mL·kg^（-1））,low-dose（5.0mL·kg^（-1）） of A.chinesis treated group and colchicine treated group.The change of liver histopathology was observed by HE and Masson staining.The mRNA of VEGF,TGF-β1 were detected by RT-PCR.The protein of Bcl-2 and Bax were detected by Western blot.In the model group liver cell obvious degeneration,necrosis,a large number of collagen fibers of the cable hyperplasia,part visible pseudolobule formation.A.chinesis large,medium,low-dose group and colchicine group liver cell degeneration and necrosis reduced A.chinesis small,medium,and highdose group was gradually reduced trend and A.chinesis large,middle dose group degree of reduction is particularly significant.Compared with model group,A.chinesis of large,medium and small dose group and colchicine group VEGF mRNA expression,A.chinesis of large,medium-dose group TGF-β1 mRNA expression reduce（P〈0.05）;compared with colchicine group,A.chinesis of large,middle dose group of VEGF mRNA expression decreased（P0.05）;A.chinesis of large,middle dose group of TGF-β1 mRNA expression decreased（P0.01）,and compared with colchicine group,large dose group of of TGF-β1 mRNA expression decreased（P〈0.05）.Compared with model group,A.chinesis of large,medium and small dose group and colchicine group Bcl-2 protein expression reduce（all is P0.05）.But A.chinesis of large,medium and small dose group and colchicine group of Bax protein expression were increased（P〈0.05）.A.chinesis regulation of VEGF,TGF-β1 may prevent the activation of hepatic stellate cells,liver
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第21期4251-4255,共5页
China Journal of Chinese Materia Medica
基金
陕西省社会发展攻关计划项目(S2011SF1610)
关键词
太白楤木
肝纤维化
凋亡
Aralia chinesis
hepatic fibrosis
apoptosis
