摘要
目的:探讨太白楤木对成纤维细胞增殖及超微结构的影响,研究太白楤木抗肝纤维化的作用机制。方法:采用体外培养的NIH3T3(小鼠胚胎成纤维细胞)作为肝星状细胞(HSC)的替代模型,常规培养,秋水仙碱、齐墩果酸及太白楤木中药血清作用于细胞,用MTT(四唑盐比色)法检测各组药物血清对NIH3T3细胞增殖的影响;用放射免疫法测定培养上清液中透明质酸(HA)的生成;电镜下观察药物血清对NIH3T3细胞超微结构的影响。结果:太白楤木、齐墩果酸、秋水仙碱血清组细胞均可抑制细胞增殖(P<0.05);显著抑制HA的合成,降低NIH3T3细胞培养上清中HA水平,与对照组比较差异有显著性意义(P<0.01),而3组之间差异无显著性意义(P>0.05)。结论:太白楤木中药血清可显著抑制NIH3T3细胞增殖和细胞外HA的合成,并可通过诱导HSC细胞凋亡来达到减少细胞数目、促进纤维化的逆转,从而达到抗肝纤维化的作用。
Objective： To study the effect of Aralia Chinesis L. on the proliferation and uhrastructure of fibroblasts and to explore the mechanisms involved in its protection against liver fibrosis. Methods： NIH3T3 fibroblasts were cultured in vitro, as the substitutive model of hepatic satellate cells （HSC）. The serum of Colchicinum or Oleanolic acid or Aralia Chinesis L. was used. The effects of the serum of them on the cell proliferation were measured by methabenzthiazuron （MTT） assay and the HA in culture medium were detected by radiommunoassay. The differences of ultrastructure of fibroblasts was observed by the electron microscope. Results ： The serums of three medicine all significantly inhibited the cell proliferation （ P 〈 0. 05 ） and the synthesis of HA as well. The content of HA in culture medium were decreased, which differed significantly from those in the control group （P 〈 0. 01 ）, but there were no evident differences between them （P 〉 0. 05 ）. Conclusion： The Aralia Chinesis L. can obviously inhibit NIH3T3 fibroblasts proliferation and decrease the synthesis of HA and induce the apoptosis of HSC, indicating the action in anti-liver fibrosis in vitro.
出处
《中西医结合肝病杂志》
CAS
2008年第2期96-98,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
陕西省中医管理局科研课题(N0.2005061)
关键词
肝纤维化
太白槐木
成纤维细胞
liver fibrosis
Aralia Chinesis L/pharmocological effect
fibroblasts
