摘要
目的:通过研究染料木素铬配合物(染料木素铬)对L-谷氨酸钠(MSG)诱导的肥胖大鼠糖代谢和脂代谢的影响,为开发治疗肥胖型2型糖尿病的新药提供依据。方法:采用MSG制备肥胖大鼠模型,将成模后的雌性大鼠(体质量超过对照组大鼠平均体质量的20%)50只随机为模型组、二甲双胍(95 mg·kg^(-1))组、低剂量(15 mg·kg^(-1))染料木素铬组、中剂量(30 mg·kg^(-1))染料木素铬组和高剂量(60 mg·kg^(-1))染料木素铬组,另取10只正常大鼠设为对照组,按上述剂量灌胃给予受试药物,每日1次,连续4周。给药结束后取静脉血,采用氧化酶法检测大鼠血清中血糖(Glu)、甘油三脂(TG)和总胆固醇(TC)水平,酶联免疫吸附测定(ELISA)法检测大鼠血清中糖化血红蛋白(GHb)、胰岛素(INS)、一氧化氮合酶(NOS)、血管紧张素Ⅱ(AngⅡ)、内皮因子1(ET-1)、前列环素2(PGI2)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平。结果:与对照组比较,给药前后其余各组大鼠体质量明显增加(P<0.05),证明造模成功;与模型组比较,给药4周后,二甲双胍组和中及高剂量染料木素铬组大鼠体质量明显降低(P<0.05);二甲双胍组和各剂量染料木素铬组大鼠血清中Glu、GHb和INS水平明显降低(P<0.05),二甲双胍组和中及高剂量染料木素铬组大鼠胰岛素敏感指数(ISI)明显升高(P<0.05),中和高剂量染料木素铬组大鼠血清中的TG、TC、NOS、AngⅡ、ET-1、TNF-α和IL-6水平明显降低(P<0.05),PGI2水平明显升高(P<0.05),二甲双胍组大鼠血清中的TC、AngⅡ、TNF-α和IL-6水平明显降低(P<0.05)。结论:染料木素铬对MSG肥胖大鼠糖代谢和脂代谢均有明显的改善作用,该作用与提高胰岛素的敏感性、改善内皮因子和抑制炎症因子功能有关。
Objective:To study the effects of genistein chromium complex genistein chromium on the glucose metabolism and lipid metabolism in the obese rats induced by monosodium L-glutamate(MSG),and to provide the evidence for developing new drugs for obese type 2 diabetes.Methods:MSG was used to estblish the obese rat models.Fifty female modeling rats(whose body mass exceeded 20%of the average body mass of the rats in control group)were randomly divided into model group,metformin group(95 mg·kg^(-1)),low dose(15 mg·kg^(-1)),medium dose(30 mg·kg^(-1))and high dose(60 mg·kg^(-1))of genistein chromium groups,another 10 normal rats were used as control group;the test drug was administered by gavage once a day for 4 weeks.After administration,the vein blood was obtained,and the levels of fasting blood glucose(Glu),triglyceride(TG)and total cholesterol(TC)in serum of the rats were measured by oxidase method;the levels of serum glycosylated hemoglobin(GHb)and insulin(INS),nitric oxide synthase(NOS),angiotensin Ⅱ(AngⅡ),endothelin-1(ET-1),prostacyclin(PGI2),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)were measured by enzyme-linked immunosorbent assay.Results:Compared with control group,the body masses of rats in the other groups were increased significantly(P<0.05)before and after administration,which proved that metformin group and the models were constructed.After 4 weeks,compared with model group,the body masses of the rats in metformin group and medium and high doses of genistein chromium groups were decreased significantly(P<0.05);the levels of Glu,GHB and INS in serum of the rats in metformin group and different doses of genistein chromium groups were decreased significantly(P<0.05);the insulin sensitivity index(ISI)of the rats in metformin group and medium and high doses of genistein chromium groups were increased significantly(P<0.05);the levels of TG,TC,NOS,AngⅡ,ET-1,TNF-α,IL-6 in serum of the rats in medium and high doses of genistein chromium groups were decreased significantly(P<0.05),and the serum PG
作者
王维纲
李晓红
刘冰
丁涛
韦康
田建明
WANG Weigang;LI Xiaohong;LIU Bing;DING Tao;WEI Kang;TIAN Jianming(Department of Traditional Chinese Medicine,Affiliated Hospital,Jilin Medical College,Jilin 132013,China;Pharmacodynamic and Toxicological Evaluation Center,Jilin College of Traditional Chinese Medicine,Changchun 130012,China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2022年第4期892-897,共6页
Journal of Jilin University：Medicine Edition
基金
国家科技部科技重大专项项目(2018ZX09711001-009-005)
吉林省科技厅重点科技攻关项目(2016020411YY)。
关键词
染料木素铬配合物
L-谷氨酸钠
糖尿病
2型
糖脂代谢指标
细胞因子
Genistein chromium complex
Monosodium L-glutamate
Diabetes mellitus,type 2
Glucose and lipid metabolism index
Cytokine
