摘要
【目的】评价小麦高温成株期(high temperature adult plant,HTAP)抗条锈病基因Yr52在生产中的应用价值,选育出农艺性状优良并高抗小麦条锈病品系,为充分利用现有高温成株期抗病资源、提高相关产量性状奠定基础。【方法】通过回交和自交结合分子标记辅助选择育种方法,将小麦抗条锈病基因Yr52转育到轮选987(LX987)、百农矮抗58(AK58)和邯6172(H6172)中。在四川绵阳和陕西杨凌鉴定圃,用小麦条锈菌流行生理小种CYR32、CYR33和CYR34混合侵染,鉴定抗病基因载体品系和受体品种以及它们的高代家系的成株期抗病性。比对中国春参考基因组,结合Yr52两翼SSR分子标记Xcfa2040(6.8 cM-Yr52)和Xbarc182(1.2 cM-Yr52),在目标基因物理区间内寻找35K SNP芯片标记,并开发成dCAPS和KASP分子标记,对BC_(2)F_(5:6)高代群体进行抗条锈病基因Yr52的检测。【结果】抗病性鉴定和农艺性状评价表明,在19个具LX987背景的BC_(2)F_(5:6)家系中,抗条锈性表现为高抗(IT=0—3,DS=1%—20%)有11个,中抗(IT=4—6,DS=15%—30%)有8个,平均千粒重(TKW)、每穗穗粒数(KPS)、单株有效分蘖(PTN)、株高(PH)和穗长(SL)分别为45.33 g、46粒、7个、113.26 cm和10.05 cm。4个具AK58背景BC_(2)F_(5:6)家系全部表现高抗(IT=0—3,DS=5%—25%);平均TKW、KPS、PTN、PH和SL分别为44.67 g、48粒、7个、96.54 cm和10.17 cm。5个具H6172背景的BC_(2)F_(5:6)家系全部表现高抗(IT=0—3,DS=5%—20%);平均TKW、KPS、PTN、PH和SL分别为43.74 g、49粒、8个、109.72 cm和10.06 cm。分子标记检测结果表明,Yr52两翼连锁的分子标记Xbarc182、Xcfa2040和Xwmc557在后代群体中的检出率分别为78.57%、66.67%和66.67%,并成功开发出1个dCAPS标记Xdcaps-Yr52-1和1个KASP标记Xkasp-Yr52-1,两者在群体中的检出率分别为73.68%和41.67%。通过比较高抗(IT=0—3)与中抗(IT=4—6)水平家系的农艺性状,结果表明,IT在0—3的家系平均TKW(P>0.05)、PTN(P>0.05)和KPS(P<0.05)
【Objective】The objective of this study is to evaluate the application value of the high temperature adult plant(HTAP)resistance gene Yr52 in wheat production for improving stripe rust resistance.And wheat lines with good agronomic characters and high disease resistance were developed and selected.It laid a foundation for making full use of the existing HTAP resistance resources and improving the yield related traits.【Method】The stripe rust resistance gene Yr52 was introgressed to Lunxuan 987(LX987),Bainong Aikang 58(AK58)and Han 6172(H6172)by backcrossing and self-crossing combined with marker-assisted selection breeding.Adult-plant resistance of donor parent,receptor cultivars and their progeny lines were evaluated in the disease nursery fields at Mianyang,Sichuan and Yangling,Shaanxi by mixed endemic physiological races CYR32,CYR33 and CYR34.In comparison to the Chinese Spring reference genome,the flanking SSR markers Xcfa2040(6.8 cm-Yr52)and Xbarc182(1.2 cm-Yr52)of Yr52 were combined to search for markers of 35K SNP chip in the physical interval of target genes,and developed into derived cleaved amplified polymorphic sequences(dCAPS)and kompetitive allele specific PCR(KASP)markers.The resistance gene Yr52was detected in BC_(2)F_(5:6)progeny lines.【Result】The evaluation of adult plant resistance and agronomic traits indicated that nineteen BC_(2)F_(5:6)lines with LX987 background was obtained:Among of them,11 were high resistance(IT=0-3,DS=1%-20%),8 were moderate resistance(IT=4-6,DS=15%-30%),the average thousand kernel weight(TKW),kernels per spike(KPS),productive tiller number per line(PTN),plant height(PH)and spike length(SL)was 45.33 g,46,7,113.26 cm and 10.05 cm,respectively.Four BC_(2)F_(5:6)families with AK58 background:all showed high resistance(IT=0-3,DS=5%-25%);the average TKW,KPS,PTN,PH and SL were 44.67 g,48,7,96.54 cm and 10.17 cm,respectively.Five BC_(2)F_(5:6)lineages with H6172 background showed high resistance to stripe rust(IT=0-3,DS=5%-20%).The average TKW,KPS,PTN,PH and SL were 4
作者
方桃红
张敏
马春花
郑晓晨
谭文静
田冉
燕琼
周新力
李鑫
杨随庄
黄可兵
王建锋
韩德俊
王晓杰
康振生
FANG TaoHong;ZHANG Min;MA ChunHua;ZHENG XiaoChen;TAN WenJing;TIAN Ran;YAN Qiong;ZHOU XinLi;LI Xin;YANG SuiZhuang;HUANG KeBing;WANG JianFeng;HAN DeJun;WANG XiaoJie;KANG ZhenSheng(Wheat Research Institute,School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan;College of Plant Protection,Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling 712100,Shaanxi;College of Agronomy,Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling 712100,Shaanxi)
出处
《中国农业科学》
CAS
CSCD
北大核心
2022年第11期2077-2091,I0001-I0006,共21页
Scientia Agricultura Sinica
基金
四川省科技厅国际科技创新合作重点研发计划(2022YFH0032)
国家自然科学基金(32101707)
西南科技大学博士基金(18zx715901)
西南科技大学博士基金(16zx7162)
龙山学术人才研究支持计划(17LZX5)。
关键词
小麦
条锈病
产量构成性状
抗病性
分子标记开发
分子标记辅助选择育种(MAS)
回交育种
Yr52
Triticum aestivum L.
stripe rust
traits of yield components
stripe rust resistance
molecular markers development
molecular marker-assisted selection(MAS)
backcross breeding
Yr52
