摘要
【目的】了解小麦品种(系)对条锈病的抗性水平及遗传多样性,掌握条锈病抗性基因的利用情况,为培育和合理利用优良小麦抗条锈病新品种提供理论依据。【方法】选用小麦条锈病流行生理小种CYR32、CYR33和CYR34对103份小麦品种(系)进行苗期抗条锈病分小种鉴定、成株期抗CYR32鉴定,并利用SSR分子标记对其进行遗传多样性分析,进而利用已知小麦抗条锈病基因Yr5、Yr9、Yr10、Yr15、Yr18和Yr26的分子标记进行抗条锈病基因检测。【结果】苗期抗性鉴定表明,103份小麦品种(系)对生理小种CYR32表现为抗病的有20份,占供试材料的19.42%;有34份品种(系)对CYR33表现为抗病,占供试材料的33.01%;36份品种(系)对CYR34表现为抗病,占供试材料的34.95%;仅有郑6辐、宁麦3号、老兰麦、京411、京作278、扬麦158共6个品种对生理小种CYR32、CYR33和CYR34均表现抗病。成株期对CYR32抗性鉴定表明郑州021等55份材料表现为成株期抗性,占供试材料的53.40%。遗传多样性分析表明,103份小麦品种(系)的遗传相似系数变异范围为0.50-0.93,平均值为0.66。103份小麦品种(系)聚类分析可分为3大类,第Ⅰ类包含4个品种,分别为花培112-2、尤皮2号、鲁沾1号和Elkhart;第Ⅱ类包含43个品种(系),其中来自于同一地区的品种(系)或含系谱来源相同的品种(系)聚在一类,说明小麦品种之间的亲缘关系与品种来源有很大的关系;第Ⅲ大类中具有相同系谱的品种(系)都聚在一个亚类,表明同一地区小麦生产品种选育过程中多数使用了相同或亲缘关系相近的材料,导致小麦育成品种之间遗传关系相近。已知抗病基因检测到含有Yr9、Yr10、Yr18和Yr26特征带的品种(系)分别有15、8、19和1份,未检测到含有Yr5和Yr15阳性条带的品种(系)。【结论】供试小麦品种(系)苗期的抗性水平较低,郑6辐、宁麦3号、老兰麦、京411、京作278、扬麦158这6份品种可能�
【Objective】The objective of this study is to understand the resistance level to stripe rust and genetic diversity of wheat cultivars(lines),master the utilization of stripe rust resistance genes,and to lay a foundation for wheat breeding and rational utilization of new cultivars with excellent resistance to stripe rust.【Method】A total of 103 wheat cultivars(lines)were identified by physiological races CYR32,CYR33 and CYR34 singly at seedling stage and CYR32 at adult stage.SSR molecular marker technique was used to evaluate the genetic diversity and the molecular markers of the important known resistance genes of wheat stripe rust Yr5,Yr9,Yr10,Yr15,Yr18 and Yr26 were used to detect the stripe rust resistance genes in the tested wheat cultivars(lines).【Result】The identification of seedling resistance showed that 20 out of 103 cultivars(lines)were resistant to physiological race CYR32,accounting for 19.42%of the tested materials;34 cultivars(lines)showed resistance to CYR33,accounting for 33.01%of the tested materials;36 cultivars(lines)showed resistance to CYR34,accounting for 34.95%of the tested materials;only six cultivars including Zheng 6 fu,Ningmai 3,Laolanmai,Jing 411,Jingzuo 278 and Yangmai 158 showed resistance to all the three physiological races CYR32,CYR33 and CYR34.The identification by CYR32 at adult stage showed that 55 cultivars(lines)such as Zhengzhou 021 had adult plant resistance,accounting for 53.40%of the tested materials.Genetic diversity analysis showed that the variation of genetic similarity coefficient of 103 cultivars(lines)ranged from 0.50 to 0.93,with an average of 0.66.Cluster analysis found that 103 cultivars(lines)were divided into three categories.The first category included four cultivars,namely Huapei 112-2,JubilejinaⅡ,Luzhan 1 and Elkhart.The second category included 43 cultivars(lines),among which the cultivars(lines)from the same region or with the same pedigree source were clustered in the same category,indicating that there was a certain relationship between the
作者
徐默然
蔺瑞明
王凤涛
冯晶
徐世昌
XU MoRan;LIN RuiMing;WANG FengTao;FENG Jing;XU ShiChang(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193)
出处
《中国农业科学》
CAS
CSCD
北大核心
2020年第4期748-760,共13页
Scientia Agricultura Sinica
基金
国家自然科学基金（31871923）
国家重点研发计划（2018YFD0200500,2016YFD0300705）
作物种质资源保护与利用专项（2019NWB036-12）
关键词
小麦条锈病
抗性鉴定
遗传多样性
Yr基因检测
wheat stripe rust
resistance identification
genetic diversity
Yr gene detection
