摘要
目的探索冬凌草甲素影响PC-3细胞迁移及侵袭的作用及机制,为冬凌草甲素治疗前列腺癌骨转移提供前期体外实验参考。方法通过CCK8检测冬凌草甲素对PC-3细胞增殖的影响,Transwell检测冬凌草甲素对PC-3细胞迁移和侵袭的影响,qRT-PCR分析冬凌草甲素溶液处理PC-3细胞后miR-204-5p及NF-κB信号通路表达情况,Western blotting分析冬凌草甲素溶液处理PC-3细胞后NF-κB信号通路蛋白表达情况。结果冬凌草甲素溶液以浓度依赖性和时间依赖性方式抑制PC-3细胞的增殖,与对照组相比差异有统计学意义(P<0.01),冬凌草甲素IC50值为10μmol/L;冬凌草甲素溶液呈浓度依赖性抑制PC-3细胞的迁移和侵袭,差异较对照组有统计学意义(P<0.01);冬凌草甲素溶液能促进PC-3细胞表达miR-204-5p并阻断NF-κB信号通路下游基因表达;过表达miR-204-5p和冬凌草甲素均能抑制PC-3细胞NF-κB信号通路下游基因表达。结论冬凌草甲素能抑制PC-3细胞的增殖、迁移及侵袭,其作用机制可能与其能上调miR-204-5p阻断NF-κB信号通路有关。
Objective To explore the effect and mechanism of oridonin on the migration and invasion of PC-3 cells,to provide preliminary in vitro experiment reference for the treatment of bone metastasis of prostate cancer with oridonin.Methods The effect of oridonin on the proliferation of PC-3 cells was detected by CCK8.The effect of oridonin on the migration and invasion of PC-3 cells was detected by the Transwell test.The expression of miR-204-5p and NF-κB signaling pathways after the treatment of PC-3 cells with oridonin was analyzed by qRT-PCR.The expression of protein in the NF-κB signaling pathway after the treatment of PC-3 cells with oridonin was analyzed by Western blotting.Results Firstly,the proliferation of PC-3 cells was inhibited by oridonin solution in a concentration-and time-dependent manner;and the difference from the control group was of statistical significance(P<0.01),the IC50 value of oridonin was 10μmol/L.Secondly,the migration and invasion of PC-3 cells were inhibited by oridonin in a concentration-dependent manner;and the difference from the control group was of statistical significance(P<0.01).Finally,the results of qRT-PCR showed that oridonin solution promoted the expression of miR-204-5p in PC-3 cells and blocked the expression of the downstream genes in the NF-κB signaling pathway;the results of Western blotting indicated that both the overexpression of miR-204-5p and oridonin could inhibit the expression of the downstream genes in NF-κB signaling pathway in PC-3 cells.Conclusions Oridonin can inhibit the proliferation,migration and invasion of PC-3 cells.Its mechanism of action may be associated with its up-regulating miR-204-5p and blocking the NF-κB signaling pathway.
作者
施浩然
包广龙
令狐熙涛
吴洪瀚
赵泽驹
刘霄
付瑶
黄帅
瓦庆德
Shi Haoran;Bao Guanglong;LingHu Xitao;Wu Honghan;Zhao Zeju;Liu Xiao;Fu Yao;Huang Shuai;Wa Qingde(Department of Orthopedics,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;Department of Urology,the Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;Department of Oncology,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;Department of Rehabilitation Medicine,West China Second University Hospital,Sichuan University,Chengdu 610041,China;Department of Orthopedics,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2022年第3期286-291,共6页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(81860322)
贵州省科技计划项目(黔科合基础-ZK[2021]重点007)
广东省基础与应用基础研究基金区域联合基金-重点项目(2020B1515120094)。
