摘要
目的探讨BRAF^(V600E)抗体通过两种不同厂家试剂、不同染色条件的手工免疫组化染色方法在甲状腺乳头状癌中的表达情况比较。方法对5例BRAF^(V600E)强阳性表达的甲状腺乳头状癌标本进行石蜡切片,根据罗氏DAB染色试剂盒(罗氏)、迈新即用型快捷免疫组化Maxvision HRP试剂盒(鼠/兔)(HRP)、即用型免疫组化Elivision Super试剂盒(鼠/兔)(ES)以及不同染色时间,将每一例样本分为9组,采用相同的修复方式和同一批号的罗氏一抗试剂,进行手工免疫组化染色实验进行对比分析。结果实验结果显示:对照组均表达为强阳性。5例实验组1和实验组2表达均为阴性。而5例实验组3和实验组4以及2例实验组5表达为弱阳性;5例实验组6和3例实验组5以及1例实验组8表达为中阳性;其余实验组7和实验组8均表达为强阳性。对8组样本的实验结果进行分析显示:实验组7和实验组8的表达强度与对照组的表达强度基本一致,但实验组7的染色时间相对较短。结论对照组BRAF^(V600E)抗体表达最理想,但染色时间长,费用也较高;手工染色中实验组7的操作简单,费用较低,同时染色效果较理想,能满足于病理诊断,可供各单位参考。
Objective Explore the BRAF^(V600E) antibody by two different manufacturers reagents,different dyeing conditions of manual immunohistochemical staining method in thyroid papillary carcinoma in the expression in comparison.Methods 5 cases of BRAF^(V600E) strong positive expression of thyroid papillary carcinoma specimens of paraffin section,according to the roche DAB staining kits(roche),Michael new ready-to-use rapid immunohistochemical Maxvision HRP kit(rat)/rabbit(HRP),ready-to-use immunohistochemical Elivision Super kit(rat)/rabbit(ES)and the different dyeing time,each sample was divided into nine groups,using the same repair methods and the same batch of roche one resistant reagent,manually immunohistochemical dyeing experiments were analyzed.Results Experimental results show that the control group were expressed as strong positive.5 cases of experimental group 1 and group 2 expression are all negative.And 5 cases of experimental group 3 and group 4 and 2 cases of experimental group 5 expressed as weak positive;5 cases of experimental group 6 and 3 cases of experimental group 5 and 1 cases in experimental group 8 expressed as positive;The rest of the group 7 and 8 are expressed as experimental group strong positive.The experimental results of 8 groups of sample analysis shows:the experimental group 7 and 8 expression of strength and controls the expression of intensity is almost the same,but the experimental group 7 dyeing time is relatively short.Conclusion Control group BRAF^(V600E) antibody expression of the ideal,but dyeing time is long,cost also is higher;Manual dyeing in the experimental group 7 of simple operation,low cost,at the same time the dyeing effect is ideal,can contented with pathological diagnosis,can be used for reference in the various units.
作者
廖子龙
张培荣
吴泽
LIAO Zi-long;ZHANG Pei-rong;WU Ze(Department of Pathology,Shenzhen Yantian People's Hospital,Shenzhen 518000,Guangdong Province,China;Department of Pathology,Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China)
出处
《罕少疾病杂志》
2022年第6期31-33,共3页
Journal of Rare and Uncommon Diseases
基金
深圳市盐田区科创局医疗卫生类科技计划项目(YTWS20210104)。
