摘要
黄酮醇合成酶(FLS)是黄酮醇合成的关键酶,对植物花色的色调和强度形成有重要作用,研究该基因密码子偏好性有助于进一步了解黄酮醇的功能.为明确罗布麻黄酮醇合成酶基因密码子的使用特性,采用CondoW、Tbtools、SPSS软件及EMBOSS在线程序分析AvFLS密码子偏好性,通过ENc绘图、中性绘图和PR2-plot分析FLS基因密码子偏好性形成的可能原因,比较AvFLS与模式生物的密码子使用频率获得最佳受体.结果表明:AvFLS的有效密码子数(ENc)、密码子适应指数(CAI)和GC含量分别为47.09、0.2和39.48%,表明AvFLS基因密码子偏好性较弱.AvFLS基因偏好性密码子有27个(RSCU>1),其中22个以A/T结尾,表明该基因偏好使用A/T结尾的密码子;基因序列进化分析及RSCU聚类分析表明,AvFLS基因的密码子使用偏好性与同源关系较近的桔梗相似;密码子碱基成分和相关性分析发现,AvFLS密码子偏好性主要受突变压力的影响;密码子使用频率比较分析发现,酵母真核表达系统更适合AvFLS异源表达.本研究表明模式植物拟南芥、烟草、番茄、甜菜和蒺藜苜蓿均可作为AvFLS的遗传转化受体,结果可为后续AvFLS改造和功能验证研究提供一定参考.(图6表3参44)
Flavonol synthase(FLS)is a key enzyme in the synthesis of flavonols.FL is an important role in the formation of the color tone and intensity of plant flowers.Studying the codon preference of this gene will help further understand the function of flavonols.To clarify the codon usage characteristics of AvFLS,CondoW,Tbtools,SPSS software,and EMBOSS online program were used to analyze the codon preference of AvFLS.Additionally,the codon preference of the FLS gene was analyzed using ENc,neutral plot,and PR2-plot possible reason.Codon usage frequencies of AvFLSs and model organisms were compared to obtain the best receptor.The effective codon number(ENC),codon adaptation index,and GC content of AvFLS were 47.09,0.2%,and 39.48%,respectively,indicating weak codon preference of AvFLS.There were 27 preferred codons in AvFLS(RSCU>1).Of these,22 codons ended with A or T,indicating that AvFLS prefers to use codons ending with A or T.Evolutionary analysis and RSCU cluster analysis of gene sequences showed that the codon use preference of AvFLS was similar to that of Eustoma russellianum with close homology.Analysis of codon base composition and correlation showed that AvFLS codon preference was mainly affected by mutation pressure;codon use frequency was relatively low.The yeast eukaryotic expression system was more suitable for the heterologous expression of AvFLS.The model plants Arabidopsis thaliana,Nicotiana tabacum,Solanum lycopersicum,Beta vulgaris,and Medicago truncatula can be used as genetic transformation receptors for AvFLS.This study provides a reference for subsequent AvFLS transformation and functional verification research.
作者
蔡德育
郭晓农
朱月滢
赵诗佳
朱玉雪
柏家林
马忠仁
CAI Deyu;GUO Xiaonong;ZHU Yueying;ZHAO Shijia;ZHU Yuxue;BAI Jialin;&MA Zhongren(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第2期423-431,共9页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31760242)
甘肃省自然科学基金项目(20JR10RA120)
西北民族大学2019年度中央高校基本科研业务费项目创新团队培育项目(31920190021)
教育部动物医学生物工程创新团队项目(IRT_17R88)
西北民族大学“双一流”引导专项生物工程特色学科项目(1001070204)
西北民族大学本科生科研创新项目(XBMU-BYL20062)资助。
