摘要
利用27对SSR分子标记对内蒙古地区13个西伯利亚杏群体的遗传多样性和遗传结构进行分析,评价其遗传多样性水平和分化程度,为内蒙古西伯利亚杏资源的合理保护与利用提供理论依据。结果显示：（1）27对SSR引物共检测到512个等位基因（Na）,各位点平均等位基因数（Na）和多态性信息含量（PIC）分别为19和0.84;物种水平上Shannons信息指数（I）和期望杂合度（He）分别为2.25和0.73。（2）群体水平上的等位基因（Na）、有效等位基因（Ne）、Shannons信息指数（I）、期望杂合度（He）和观察杂合度（Ho）分别为6.95、4.48、1.60、0.76和0.56;其中科左后旗群体遗传多样性最丰富,克什克腾旗群体遗传多样性最低。（3）基于F统计量分析的遗传分化系数（Fst）为0.12,基因流（Nm）为1.86;分子方差分析显示内蒙古西伯利亚杏群体大部分遗传变异来自群体内（92%）,群体间的遗传变异仅占8%。（4）内蒙古西伯利亚杏群体遗传距离为0.04～0.67,遗传相似度为0.33～0.83;遗传相似度的聚类分析、遗传距离的主坐标分析和遗传结构分析（Structure）均将供试13个群体划分为4组。Mantel检测显示,内蒙古西伯利亚杏群体遗传距离与地理距离呈显著相关关系（r=0.453,P〈0.01）。研究表明,内蒙古西伯利亚杏资源具有丰富的遗传多样性,这一特性与其群体的大小、悠久的演化历史以及自身生物学特性相关;内蒙古西伯利亚杏群体间存在中等程度的遗传分化,这可能源于自然地理隔离以及近期人类活动引起的生境片段化。
In order to provide theoretical support for reasonable protection and utilization of Siberian apricot resources, we analyzed the genetic diversity and population structure of 13 Siberian apricot populations in Inner Mongolia through twenty seven pairs of SSR markers. The results showed that： （1） a total of 512 alleles （Na） were identified based on 27 SSR molecular markers, the average number of alleles （Na） per locus and polymorphism information content （PIC） per locus were 19 and 0.84, respectively. A high level of genetic diversity was revealed by Shannons information index （I, 2.25） and expected heterozygosity （He, 0.73） at species level. （2） The average number of alleles （Na）, number of effective alleles （Ne）, Shannons information index （I）, expected heterozygosity （He） and observed heterozygosity （Ho） were 6.95, 4.48, 1.60, 0.76 and 0.56, respectively at population level. Comprehensive analysis suggested that Kezuohouqi population exhibits the highest level of genetic diversity, whereas the population of Keshiketengqi exhibits the lowest level of genetic diversity. （3） The genetic differentiation coefficient （Fst） based on F statistic and gene flow （Nm） were 0.12 and 1.86, respectively; Analysis of molecular variance showed that the most of the genetic variation within population （92%）, more than that among populations （8%）. （4） The genetic distance among populations in Inner Mongolia Siberian apricot ranged from 0.04 to 0.67, the genetic identity among populations in Inner Mongolia Siberian apricot ranged from 0.33 to 0.83. UPGMA cluster analysis, principal coordinates analysis （PCoA） and structure analysis showed that 13 populations could be divided into four clusters. The Mantel test showed that the genetic distance among the populations was significantly correlated with geographic distance （r=0.453, P〈 0.01）. All the results supported that the genetic diversity of Siberian apricot in Inner Mongolia was relatively ab
出处
《西北植物学报》
CAS
CSCD
北大核心
2016年第11期2182-2191,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家十二五课题支撑(2013BAD14B02)
