摘要
西伯利亚杏是东北地区重要的果树和观赏植物资源,通过ISSR分子标记技术对东北地区2个西伯利亚杏自然居群的遗传多样性进行分析评价。根据多态性条带百分比(PPL)、Nei's基因多样性指数(H)、Shannon信息指数(I)评价遗传多样性的大小,对西伯利亚杏个体和居群水平上的遗传多样性进行分析。结果表明:西伯利亚杏个体水平遗传多样性高于居群水平。居群水平上,通榆居群的遗传多样性较高;遗传变异主要存在于居群内部,且在居群内部分布较均匀。81份西伯利亚杏依据遗传相似系数构建的聚类分析图和PCA主成分分析图,二者研究结果一致,认为通榆居群的遗传多样性较高,东宁居群的西伯利亚杏可能是从通榆居群漂移过去的,并产生了适应当地生境的变异类型。因此认为东北西伯利亚杏保护的重点应是通榆居群以及东宁居群中的变异类型。
A.sibirica is an important resource of fruit trees and ornamental plants in northeast China.Using the technology of molecular markers of inter-simple sequence repeat（ISSR）,the genetic diversity of two A.sibirica populations in northeast China was analyzed and evaluated.According to the percentage of polymorphic bands（PPL）,Nei＇s genetic diversity index（H） and Shannon information index（I）,the genetic diversity in the level of individuals and populations of A.sibirica were analyzed.The results showed that genetic diversity on individual level was higher than population level.The genetic diversity of Tongyu population was higher on population level.The genetic variation mainly existed inside the populations and was uniformly distributed in each population.Both the results of dendrogram and PCA principal component analyses based on genetic similarity coefficient for81 samples of A.sibirica were unanimous.The genetic diversity of Tongyu population resource was higher.A.sibirica of Dongning population resources probably originated from Tongyu population,and then variation types emerged to adapt to the local habitat.Based on the above conclusions,the priority of conservation should be given to Tongyu population,and to variation types in Dongning population.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2014年第3期300-305,共6页
Journal of Jilin Agricultural University
基金
公益性行业(农业)科研专项(201003058)
