摘要
目的探讨毛冬青三萜皂苷(IPTS)对动脉粥样硬化(AS)模型大鼠粪便和尿液代谢组学的影响。方法采用高脂饮食联合腹腔注射维生素D3诱导大鼠AS模型,实验分为对照组、模型组、IPTS组。检测大鼠血清中血脂四项水平,应用核磁共振(NMR)技术对粪样和尿样进行代谢组学分析。结果IPTS干预后,大鼠血清TG含量显著下降(P<0.05),HDL-C含量显著升高(P<0.05)。代谢组学分析结果表明,模型组大鼠中上调的差异代谢产物主要包括氨基酸类(亮氨酸、酮亮氨酸、胍基乙酸酯)和肠道菌群相关代谢物(胆碱、甜菜碱、氧化三甲胺等),下调的代谢产物包括三羧酸循环产物(琥珀酸盐、富马酸盐和戊二酸)、核苷酸类(腺嘌呤、黄嘌呤等)以及氨基酸类(色氨酸、犬尿氨酸等),经IPTS干预后,部分三羧酸循环产物、氨基酸和核苷酸代谢产物以及肠道菌群代谢物得到了回调。结论IPTS对部分粪便和尿液差异代谢物有调节改善作用,对AS大鼠的代谢紊乱一定的预防和改善作用。
OBJECTIVE To investigate the effect of Ilex pubescens triterpene saponins(IPTS)on fecal and urine metabolomics in atherosclerotic(AS)rats.METHODS AS model rats were induced by high-fat diet combined with intraperitoneal injection of vitamin D3.The rats were divided into control group,model group and IPTS group.The contents of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)in the serum were tested.The fecal and urine samples of the rats were taken for metabolomic analysis using nuclear magnetic resonance(NMR)technology.RESULTS After administration of IPTS,the content of TG significantly decreased(P<0.05),and the content of HDL-C significantly increased(P<0.05)in rat serum.The result of metabonomics analysis showed that the differential metabolites up-regulated in the model group mainly included amino acids(leucine,ketooleucine,guanidoacetate)and intestinal flora-related metabolites(choline,betaine,trimethylamine oxide,etc.).The down-regulated metabolites included the tricarboxylic acid cycle products(succinate,fumarate and glutarate),nucleotides(adenine,scutellaria,etc.)and amino acids(tryptophan,kynurenine,etc.),which showed that the energy metabolism,amino acid metabolism,nucleotide metabolism and intestinal flora-host co-metabolism of the model group rats were disturbed.After administration of IPTS,some products of TCA cycle,nucleotide metabolites,amino acid metabolites,and metabolites of gut microbiota were corrected,which indicated IPTS could ameliorate the metabolic disorders caused by atherosclerosis.CONCLUSION IPTS can partly regulate the differential metabolites of fecal and urine samples in AS rats and have a good therapeutic effect on AS rats.
作者
白荣钰
易欢
邱静文
陈丰连
王莹
陈冰莹
李瑜
张蕾
BAI Rong-yu;YI Huan;QIU Jing-wen;CHEN Feng-lian;WANG Ying;CHEN Bing-ying;LI Yu;ZHANG Lei(School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Nursing, Guangzhou University of Chinese Medicine, Guangzhou 510006, China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2022年第5期424-431,共8页
Journal of Nanjing University of Traditional Chinese Medicine
基金
广东省教育厅创新强校工程项目(2016KTSCX019)
广东省科技计划项目(2016A020226031)
广州市科技局一般科学研究项目(201607010334)。
关键词
毛冬青
三萜皂苷
动脉粥样硬化
代谢组学
粪便
尿液
Ilex pubescens
triterpene saponins
atherosclerosis
metabolomics
feces
urine
