摘要
背景:基于网络药理学方法发现瓜蒌-薤白药对中主要生物活性化合物对冠心病有多功能作用;然而其治疗冠心病的机制尚未得到充分阐释。目的:探讨瓜蒌-薤白通过调节肠道微生物的组成改善冠心病的作用及机制。方法:40只SD大鼠按随机数字表法分为空白组、模型组、阳性药组、药对组,除空白组外,其余大鼠连续灌胃脂肪乳剂和注射垂体后叶素制备冠心病大鼠模型。造模后模型组大鼠灌胃蒸馏水(10 mL/kg)进行对照,阳性药组大鼠每日灌胃辛伐他汀4 mg/kg,药对组每日灌胃瓜蒌-薤白7.56 g/kg,各组大鼠给药均为14 d。观察大鼠心电图、心肌病理及血脂变化,并通过16S rDNA测序技术研究大鼠经干预后肠道微生物结构。结果与结论:①心电图结果显示模型组ST抬高;阳性药组与药对组心电图无明显问题。②与空白组相比,模型组总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平均显著增高(P<0.05);与模型组相比,阳性药组、药对组总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平均显著下降(P<0.05)。③与空白组相比,模型组局灶心肌细胞坏死;阳性药组、药对组部分心肌细胞走行紊乱。④与空白组比较,模型组、药对组及阳性药组的Ace、Shannon、Chao指数升高(P<0.05),Simpson指数降低(P<0.05);与模型组比较,阳性药组、药对组的Ace、Chao指数降低(P<0.05),Shannon指数无差异(P>0.05),Simpson指数降低(P<0.05)。⑤与空白组相比,模型组Desulfovibrionia、Muribaculaceae_norank等相对丰度升高,Clostridia、[Eubacterium]_coprostanoligenes_group_norank等相对丰度降低;与模型组相比,药对组WPS-2_norank、Muribaculaceae_norank等相对丰度升高,Clostridia、[Eubacterium]_coprostanoligenes_group_norank等相对丰度降低;阳性药组Desulfobacterota、[Eubacterium]_coprostanoligenes_group_norank等相对丰度升高,Firmicutes、Muribaculaceae_norank等相对丰度降低;与阳性药相比,药
BACKGROUND:A network-based pharmacological approach has identified multifunctional effects of the main bioactive compounds in the Trichosanthis Fructus-Allii Macrostemonis Bulbus on coronary heart disease;however,the mechanism of its therapeutic effect on coronary heart disease has not been fully elucidated.OBJECTIVE:To investigate the role and mechanism of Trichosanthis Fructus-Allii Macrostemonis Bulbus in improving coronary heart disease by regulating the composition of gut microbiota.METHODS:Forty Sprague-Dawley rats were randomly divided into four groups:blank control group(n=10),model group(n=10),positive drug group(n=10),and medicine pair group(n=10).A rat model of coronary heart disease was established by continuous gastric perfusion of fat emulsion and injection of pituitrin.After modeling,rats in the model group were gavaged with distilled water(10 mL/kg)for control,rats in the positive drug group were gavaged with simvastatin 4 mg/kg per day,and rats in the medicine pair group were gavaged with Trichosanthis Fructus-Allii Macrostemonis Bulbus pairs 7.56 g/kg per day.All interventions lasted for 14 days.Electrocardiograms and myocardial pathology were observed,and blood lipid levels were measured.The structure of gut microbiota was analyzed using 16S rDNA sequencing technology.RESULTS AND CONCLUSION:Electrocardiogram results showed ST segment elevation in the model group.There were no significant abnormalities in the electrocardiograms of the positive drug group and medicine pair group.Compared with the blank control group,the levels of total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol were significantly higher in the model group(P<0.05).Compared with the model group,the levels of total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol were significantly lower in the positive drug group and medicine pair group(P<0.05).Compared with the blank control group,focal myocardial cell necrosis was observed in the model group,while partial myocardial cell disarray was ob
作者
孙广瀚
谢珍聪
孙咪
徐洋
郭栋
Sun Guanghan;Xie Zhencong;Sun Mi;Xu Yang;Guo Dong(The First Clinical Medical College of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China;Teacher Development Center,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong Province,China;Institute of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2025年第5期917-927,共11页
Chinese Journal of Tissue Engineering Research
基金
国家中医药管理局中医全科医学重点学科项目(220254),项目负责人:郭栋。
关键词
“瓜蒌-薤白”药对
16S
rDNA
冠心病
肠道菌群
大鼠
Trichosanthis Fructus-Allii Macrostemonis Bulbus
16S rDNA
coronary heart disease
gut microbiota
rat
