摘要
本研究探讨了α-松油醇对指状青霉抑霉唑抗性菌株Pdw03的抑制作用及其抑菌机理。体外实验结果表明α-松油醇抑制Pdw03生长的最小抑菌浓度(minimal inhibitory concentration,MIC)和最小杀菌浓度(minimal fungicidal concentration,MFC)均为4.00μL/mL;体内实验研究发现MFC和5 MFCα-松油醇可明显降低接种Pdw03椪柑果实的绿霉病发病率和发病直径;碘化丙啶(propidium iodide,PI)染色实验发现,经1/2 MICα-松油醇处理后,Pdw03的细胞膜完整性降低,麦角固醇含量显著降低(P<0.05),麦角固醇合成相关基因ERG9表达量显著下调(P<0.05)。综上,α-松油醇通过抑制ERG9基因的表达影响菌株Pdw03的生长,可作为采后柑橘病害防治的有效替代品。
This study explored the inhibitory effect and potential antifungal mechanism ofα-terpineol against an imazalil-resistant strain of Penicillium digitatum Pdw03.In vitro experiment showed thatα-terpineol exhibited antifungal activity against P.digitatum Pdw03,with minimum inhibitory concentration(MIC)and minimum fungicidal concentration(MFC)of 4.00μL/mL.In vivo results showed thatα-terpineol at MFC and 5 MFC significantly reduced the incidence of green mold and lesion diameter in ponkan fruit inoculated with P.digitatum Pdw03.Propidium iodide(PI)staining indicated that 1/2 MICα-terpineol evidently decreased the cell membrane integrity,ergosterol content(P<0.05)and gene expression of squalene synthase(ERG9)(P<0.05)in P.digitatum Pdw03.These results suggest thatα-terpineol affects the growth of P.digitatum Pdw03 by inhibiting the gene expression of ERG9,and thus is an effective alternative for controlling postharvest citrus diseases.
作者
欧阳秋丽
刘洋梅
陈悦
陶能国
OUYANG Qiuli;LIU Yangmei;CHEN Yue;TAO Nengguo(College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第7期8-13,共6页
Food Science
基金
国家自然科学基金面上项目(31772364)
湖南省教育厅重点项目(19A476)
湖南省自然科学基金青年科学基金项目(2020JJ5536)。
