摘要
目的:探讨内毒素通过干预大鼠玻璃体中晶状体蛋白家族蛋白表达影响视网膜电图波幅和玻璃体通透性的机制。方法:将糖尿病视网膜病变大鼠(n=36)随机平分为三组:模型组、内毒素A组、内毒素B组,每组12只。内毒素A组、内毒素B组麻醉扩瞳后分别给予玻璃体腔注射30 ng/ml或60 ng/ml内毒素1μl,模型组注射同等体积的0.9%氯化钠溶液,1次/周,持续4周。结果:内毒素A组、内毒素B组处理第1、2、4周体重低于模型组(均P<0.05),且内毒素B组低于内毒素A组(P<0.05)。内毒素A组、内毒素B组处理第1、2、4周的血糖高于模型组(均P<0.05),且内毒素B组高于内毒素A组(P<0.05)。内毒素A组、内毒素B组处理第1、2、4周的视网膜电图a波、b波波幅低于模型组(均P<0.05),且内毒素B组低于内毒素A组(P<0.05)。内毒素A组、内毒素B组处理第4周的视网膜周细胞计数低于模型组(均P<0.05),内皮细胞计数高于模型组(均P<0.05),且内毒素A组与内毒素B组对比差异有统计学意义(P<0.05)。内毒素A组、内毒素B组处理第4周的眼球组织血管内皮细胞生长因子(VEGF)与血管内皮细胞生长因子受体2(VEGFR2)相对表达水平高于模型组(均P<0.05),且内毒素B组高于内毒素A组(P<0.05)。结论:内毒素可上调糖尿病视网膜病变大鼠的玻璃体中晶状体蛋白家族蛋白VEGF与VEGFR2表达水平,影响玻璃体的通透性,降低视网膜电图波幅,可导致糖尿病视网膜病变大鼠体重降低,促使血糖持续升高。
Objective:To explore the mechanism by which endotoxin interferes with the expression of crystallin family proteins in rat vitreous to affect electroretinogram amplitude and vitreous permeability.Methods:Diabetic retinopathy rats(n=36)were randomly divided into model group,endotoxin group A,endotoxin group B,with 12 rats in each group.The endotoxin group A and endotoxin group B were given intravitreal injections of 30 ng/ml or 60 ng/ml endotoxin 1μl after anaesthesia and pupil dilation.The model group was injected with the same volume of normal saline,once a week for 4 weeks.Results:The body weight of endotoxin group A and endotoxin group B was lower than that of the model group at 1st,2nd and 4th week,and the endotoxin group B was lower than the endotoxin group A(all P<0.05).The blood glucose of endotoxin group A and endotoxin group B was higher than that of the model group at the 1st,2nd and 4th week,and the endotoxin group B was higher than that of endotoxin group A(all P<0.05).The a-wave and b-wave amplitudes of the electroretinogram at 1st,2^(nd) and 4th week in endotoxin group A and endotoxin group B were lower than those of the model group,and the endotoxin group B was lower than the endotoxin group A(all P<0.05).The retinal pericyte count of endotoxin group A and endotoxin group group B was lower than that of model group at 4th week,and the endothelial cell count was higher than that of model group,and the difference between endotoxin group A and endotoxin group B was statistically significant(all P<0.05).The vascular endothelial growth factor(VEGF)and Vascular endothelial growth factor receptor 2(VEGFR2)of the eyeball tissues of endotoxin group A and endotoxin group B were higher than those of model group at 4th week,and the endotoxin group B was higher than the endotoxin group A(all P<0.05).Conclusion:Endotoxin can up-regulate the expression levels of crystallin family proteins-VEGF and VEGFR2 in the vitreous of diabetic retina rats,affect the permeability of the vitreous body,reduce the amplitude of th
作者
薛涛
张娟娟
杜建英
马文婷
董永孝
XUE Tao;ZHANG Juanjuan;DU Jianying;MA Wenting;DONG Yongxiao(Department of Ophthalmology,the First People’s Hospital of Xianyang,Xianyang 712000,China)
出处
《陕西医学杂志》
CAS
2022年第5期543-546,565,共5页
Shaanxi Medical Journal
基金
陕西省卫生计生科研基金资助项目(2016E007)。
