摘要
考察艾叶乙醇提取物对万古霉素(VAN)诱导的急性肾损伤(AKI)的保护作用及其机制。采用小鼠腹腔注射VAN建立药物性AKI模型,30只实验小鼠分成空白组、模型组、高、中、低3个给药剂量组,并采用灌胃方式给药(ig)。检测小鼠血清中肾功能指标小鼠胱抑素C(Cys C)、尿素氮(BUN)、肌酐(Scr)的含量水平,氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶活性(GSH-Px)的含量水平,以及炎症指标肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、超敏C-反应蛋白(hs-CRP)含量水平;此外,观察染色肾脏病理情况,并检测肾组织匀浆中细胞凋亡相关基因Bax、Bcl-2、Caspase-3的含量水平。结果表明,艾叶乙醇提取物对VAN诱导的AKI具有保护作用,其机制可能与改善氧化应激、抑制炎症与细胞凋亡等有关。
The goal of the study was to investigate the protective effect and mechanism of Artemisia argyi ethanol extract on chemotherapeutic vancomycin(VAN)-induced acute kidney injury(AKI).The acute kidney injury model of male ICR mice was induced by intraperitoneal injection(ip)of VAN.Thirty mice were divided into the blank group,model group,high dose group,middle dose group and low dose group,which were given medicine by gastric perfusion(ig).Serum levels of cystain C(Cys C),blood urea nitrogen(BUN)and serum creatinine(Scr)were measured,which could reflect renal function of mice.Serum oxidative stress and inflammation indices were also determined,including muscular dystrophy association(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),tumor necrosis factor-alpha(TNF-α),Interleukin-1β(IL-1β),and high-sensitive c-reactive protein(hs-CRP).In addition,hematoxylin-eosin staining(HE)was employed for measuring the damage of renal tissues and the content of apoptosis b-cell lymphoma-2(Bcl-2),Bcl-2 assaciated X protein(Bax)and caspase-3 were measured too.All results showed that Artemisia argyi extract exhibits protective effect on chemotherapeutic VAN-induced AKI,whose mechanism could be related to the oxidative stress,inflammatory reaction and apoptosis.
作者
李达
李敏
蔡文志
柳文媛
徐健
杨宝卫
冯锋
LI Da;LI Min;CAI Wenzhi;LIU Wenyuan;XU Jian;YANG Baowei;FENG Feng(Jiangsu Food and Pharmaceutical Science College,Huai'an 223003;Changzhou Maternal and Child Health Care Hospital,Changzhou Medical Center,Nanjing Medical University,Changzhou 213000;Department of Pharmaceutical Analysis,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2022年第2期239-244,共6页
Journal of China Pharmaceutical University
基金
江苏省高等学校自然科学研究面上资助项目(No.21KJD350001)
淮安市自然科学研究计划(指导性)资助项目(No.HABZ202028)。
