摘要
目的:探讨紫苏粗提物对酶的抑制作用,阐明其对代谢综合征的药效物质基础。方法:测定紫苏粗提物中黄酮及酚酸含量;以抑制率为指标,测定紫苏粗提物对不同酶活性的抑制作用;采用分子对接预测紫苏粗提物对胰脂肪酶和淀粉酶抑制作用靶点。结果:紫苏粗提物中黄酮含量为0.3098 mg/mg,总酚酸含量为58.59 mg/g。紫苏粗提物对α-淀粉酶、黄嘌呤氧化酶、胰脂肪酶及乙酰胆碱酯酶的IC_(50)值分别为0.273,0.244,0.347,0.018 mg/mL。芹菜素-5-O-β-D-葡萄糖苷、木犀草素-7-二葡萄糖苷酸、芹菜素-7-葡萄糖苷、山奈酚-3-O-芸香苷、迷迭香酸、芦丁等为紫苏粗提物的有效活性成分。结论:紫苏粗提物对4种酶都有一定的抑制活性,且呈剂量—效应关系。
Objective:The research is to explore the enzyme inhibition of Perilla crude extract,and to preliminarily elucidate the pharmacological substance basis of them on hyperglycemia,hyperuric acid and hyperlipidemia.Methods:The content of flavonoids and phenolic acid in UV extract was determined and showed the active components associated with enzyme inhibition.In vitro experiments,the inhibition of Perilla extracts on different enzyme activity was determined and the inhibitory activity was evaluated by IC_(50).Molecular docking was used to predict the targets of Perilla crude extract on pancreatic lipase and amylase inhibition.Results:Flavone content in the crude UV extract was 0.3098 mg/mg,and the total phenolic acid content was 58.59 mg/g.The IC_(50) values of Perilla crude extractd forα-amylase,xanthine oxidase,pancreatic lipase and acetylcholinesterase were 0.273,0.244,0.347 and 0.018 mg/mL,respectively.Apigenin-5-O-β-D-glucosine,xyrhinin-7-diglucosotide,apigenin-7-glucosine,cannol-3-O-ruassin,rosemary acid,rutin and others were the effective active components of perilla crude extracts.Conclusion:The Perilla crude extracts all have certain inhibitory activities on four enzymes,and presents a dose-effect relationship.
作者
余雨婷
张彦
张迎
李娜娜
程研
郑梦迪
闫平
柴希艳
YU Yu-ting;ZHANG Yan l;ZHANG Ying;LI Na-na;CHENG Yan;ZHENG Meng-di;YAN Ping;CHAI Xi-yan(College of Pharmacy,'Medical University,',Shaanxi 710021,China;Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China;'Yurun Baide Healthy Management Co.,Ltd.,',Shaanxi 710061,China)
出处
《食品与机械》
北大核心
2022年第4期183-188,共6页
Food and Machinery
基金
陕西省科协提升公共素质项目(编号:2021PSL43)
榆林市科技局项目(编号:CXY-2020-092)
西安医学院省级大学生创新创业训练项目(编号:S202111840054)。
关键词
代谢综合征
紫苏
黄酮
总酚酸
酶抑制剂
分子对接
metabolic syndrome
Perilla frutescens L.Britt.
flavonoids
the total phenolic acid
enzymatic inhibitors
molecular docking
